نتایج جستجو برای: percoll gradient

تعداد نتایج: 126822  

2014
Nutthee AM-IN Vibuntita CHANKITISAKUL Mongkol TECHAKUMPHU

The aim of this study was to evaluate sperm quality following separation and selection using a silane-coated silica colloid method (PureSperm ® ) or Percoll ® . Frozen/thawed sperm from buffalo bulls (n=5) were assigned to two groups. The first group was separated using a Percoll ® discontinuous gradient (45 and 90%) and the second group by a single layer of PureSperm ® . Sperm motility, concen...

Journal: :Journal of Assisted Reproduction and Genetics 1997

Journal: :Reproduction, nutrition, development 1993
S Hamamah M Lanson C Barthelemy M A Garrigue J Lansac J P Muh D Royere

In order to evaluate the role of human follicular fluid (HFF) on the fertilizing capacity of spermatozoa, we studied the effect of HFF on the lipid composition and on the movement characteristics of human spermatozoa. Spermatozoa (spz) from normospermic patients were prepared with a discontinuous Percoll gradient and incubated in Ménézo B2 medium with or without a supplement of 20% HFF (HFF-Per...

Journal: :Analytical biochemistry 1996
S L Abrahamse R J Bridges M D De Jong R J Bindels C H Van Os

Surface and crypt cells of rabbit distal colon were separately isolated, and amiloride-sensitive 22Na+ uptake could only be demonstrated in a crude membrane fraction derived from surface cells. For purification of apical membranes of surface and crypt cells (H(+)-K+)-ATPase and alkaline phosphatase were used as putative apical membrane markers. Apical membranes of surface cells were isolated af...

Journal: :Clinical chemistry 1985
D B Milne N V Ralston J C Wallwork

Platelets, mononucleated cells, polymorphonucleated cells, and erythrocytes were separated from whole blood by use of discontinuous gradients of colloidal polyvinylpyrrolidone-coated silica ("Percoll"). We measured the zinc content of these cells by flame atomic absorption spectrophotometry, using a modified technique for micro-samples that obviated matrix interferences. Thus, results obtained ...

2017
Moeko Sato Takeshi Hatta Hiroki Maeda Daigo Tsubokawa M Abdul Alim Naotoshi Tsuji Noboru Inoue Hiroshi Suzuki Rika Umemiya-Shirafuji

In this study, potential of the Percoll density gradient centrifugation method for separation of bovine red bold cells (RBCs) infected with each merozoite type of Babesia ovata was examined. By the Percoll density gradient centrifugation, bovine RBCs infected with active forms of B. ovata merozoites were essentially collected from one of the layers. Moreover, this method made it possible to eli...

Journal: :Plant physiology 1982
Z Liang C Yu A H Huang

A procedure for isolating spinach (Spinacia oleracea L.) leaf peroxisomes in 0.25 molar sucrose solution by Percoll density gradient centrifugation followed by removal of the Percoll by washing and centrifugation was established. The preparation contains more than 90% peroxisomes as intact organelles with no detectable chlorophyll or cytochrome oxidase contamination. The peroxisomes are stable ...

Journal: :Plant physiology 1982
K J Talbot P J Russell

A procedure has been developed using Percoll density gradients for the isolation and purification of nuclei from germinated conidia of wild-type Neurospora crassa St. Lawrence strain 74A. Crude nuclei were purified isopycnically in gradients of Percoll, which is silica coated with polyvinylpyrrolidone. A DNA:RNA:protein ratio of 1:3.5:6.5 was found in purified nuclei. Cytoplasmic contamination ...

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