background aflatoxins are a group of very toxic compounds produced by a fungal species and are found in food products. aflatoxin m1 (afm1) is a type found in dairy products and resistant to pasteurization. afm1 can adversely affect hepatocytes in human and leading to various liver diseases. thus, it is essential to examine raw milk for the presence of afm1. conclusions according to the results ...

dispersive liquid-liquid microextraction method (dllme) combined with high-performance liquid chromatography-ultraviolet detection (hplc-uv) was used to determine thiamine (b1), nicotinamide (b3) and pyridoxine (b6) in sour cherry juice. this method was rapid, simple and sensitive. separation was accomplished using a c18 column. the optimum chromatographic conditions were found to be: mobile ph...

background & aim: according to the increase in diseases such as diabetes and obesity, using of saccharose alternatives for producing of low-calorie and sugar-free alternatives, are taken into consideration of researchers and industrialists. among them, steviosidewith the 300 times more sweetness than saccharose has special place, due to is a natural. the aim of this study is optimizing of stevi...

determination of drug residues in food is of great importance due to their toxicity. long-term exposure with low level of drug residues could be important, especially in children. based on document study, oxytetracycline (otc) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...

this study a novel high performance liquid chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using box–behnken experimental design. the method was performed in the isocratic mode on a rp-18 column (30 °c) using a mobile phase consisting of 0.05 m ammonium acetate buffer/ methanol mixture (30:70) and a flow-rate of 1.0 ml/min. the method was valida...

purpose : fluoroquinolones are widely used antibiotic for prophylaxis of intra and postoperative infections in individuals undergoing cataract surgery. this study was designed to assess the penetration of ciprofloxacin into the ocular aqueous humor (topical only versus topical and oral administration). methods : studied population (n=47) consisted of two groups: group one (n=26) and group two (...

objective(s) a sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (fmoc-cl) is described. materials and methods the method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes wi...

5-hydroxymethylfurfural (5-hmf) is known as an indicator of quality deterioration in a wide range of foods. the current study covered 70 samples taken from domestically produced foods and drinks available on the iranian markets (including honey, jam, fruit cakes tomato paste, ketchup, syrup, fruit juice, canned fruit, uht milk, instant coffee and jelly powder). hmf levels were determined using ...

the present study was aimed to develop and validate a high performace liquid chromatography method for determination of thiocolchicoside and dexketoprofen trometamol in combined dosage form. the proposed hplc method utilizes an agilent eclipse c-8 column (5 µm, 250×4.6 mm) at ambient temperature. a 23 full factorial design was performed for optimization of mobile phase for separation of two dru...

an agar well diffusion bioassay method for determination of clarithromycin in human plasma, using micrococcus luteus atcc 9341 as the assay organism, was compared with a selective high-performance liquid chromatographic (hplc) method with uv detection. spiked plasma was used to prepare standard and control samples for both methods. the results of the bioassay analyses with spiked plasma samples...