white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including iran. in the present study, a pair of primers was designed according to the sequence of vp 28 gene of wssv in the genbank. vp28 gene from an iranian wssv isolate (irvp28) was cloned, sequenced and expressed in escherichia coli bl21(de3) strain in order to produce vp28 protein...

Background: Toxoplasma gondii is an obligate intracellular protozoan parasite which has significant medical and veterinary impact on all around the world. Tracking of specific antigens or antibodies for toxoplasmosis is the main choice in its diagnosis. Recombinant proteins will improve sensitivity and specificity and reduce problems of standardization and reproducibility of diagnostic kits. To...

pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. this bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: a and b. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of p. aer...

BACKGROUND Microbial lipases particularly Pseudomonas lipases are widely used for biotechnological applications. It is a meaningful work to design experiments to obtain high-level active lipase. There is a limiting factor for functional overexpression of the Pseudomonas lipase that a chaperone is necessary for effective folding. As previously reported, several methods had been used to resolve t...

Background: Tuberculosis (TB) is among the deadliest diseases and a significant cause of illness across globe. Several studies on mycobacterial proteins, such as proteases transporters that are essential for survival pathogenesis have aimed to develop an efficient anti-tubercular agent. In mycobacterium, lysine exporter (LysE) amino acid transporter probable target a...

Interleukin (IL)-13 plays an important role in the pathogenesis of asthma. A polymorphic variant of human IL-13 R130Q, results in substitution of an arginine with a glutamine was shown to be associated with asthma in Chinese Han nationality. We examined the functional consequences of this variant in vitro to investigate whether this variant enhanced functional activity compared with wild type I...

Introduction: Pathogenic strains of Proteus mirabilis have important roles in urinary tract infection. Proteus toxic agglutinin (Pta) is amongst the most important virulence factors of P. mirabilis. This protein has a conserved sequence present in all the strains which could be evaluated as a novel vaccine target against them. The aims of the current study were the expression, purification and ...

Background and Objectives Polytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several immunogenic epitopes, inducing immune responses. Materials and Methods In this study, a DNA construct comprising of sequences encoding ...

Globally, Brucella melitensis and B. abortus are the most common cause of human brucellosis. The outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens which are considered as potential vaccine candidates. We aimed to design the fusion protein from Brucella L7/L12 and truncated Omp31proteins, in silico, clone the fusion in p...