نتایج جستجو برای: pfa

تعداد نتایج: 1196  

2018
Katharina N Richter Natalia H Revelo Katharina J Seitz Martin S Helm Deblina Sarkar Rebecca S Saleeb Elisa D'Este Jessica Eberle Eva Wagner Christian Vogl Diana F Lazaro Frank Richter Javier Coy-Vergara Giovanna Coceano Edward S Boyden Rory R Duncan Stefan W Hell Marcel A Lauterbach Stephan E Lehnart Tobias Moser Tiago F Outeiro Peter Rehling Blanche Schwappach Ilaria Testa Bolek Zapiec Silvio O Rizzoli

Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA Despite being less toxic than PFA, and, as most aldehydes, likely usable as a fixative, glyoxal has not yet bee...

2007
P. B

PFA was not different with shaded vs. exposed limbs of the same trees nor between shaded interior trees and outside ones with high light exposure. Tehama trees expressed lower PFA than adjacent Serre PFA of Tehama was also low at a site where Serr trees (removed 1982) had very high drop the previous three years. Fruitfulness of all monitored limbs in 1982 ranged from 6 to 97% and the earlier co...

Journal: :Arteriosclerosis, thrombosis, and vascular biology 2009
Ricardo Villa-Bellosta Víctor Sorribas

OBJECTIVE The role of inorganic phosphate in the pathogenesis of vascular calcification (VC) has been studied extensively in recent years. Phosphonoformic acid (PFA), an inhibitor of type II Pi transporters, has been traditionally used to study the involvement of Pi transport in VC, because PFA also prevents calcium deposition in vitro. However, aortic vascular smooth muscle cells (VSMCs) only ...

2012
Ralf Karger Karoline Reuter Jochen Rohlfs Christopher Nimsky Ulrich Sure Volker Kretschmer

We investigated whether the inclusion of the PFA-100 in the preoperative screening of neurosurgical patients might reduce perioperative bleeding complications. Patients with intracranial space-occupying lesions who were scheduled for neurosurgery underwent routine preoperative PFA-100 testing. In case of an abnormal PFA test, patients received prophylactic treatment with desmopressin. 93 consec...

2011
Justin Tatch Moore Giorgio Venturi

In these notes we will present an exposition of the Proper Forcing Axiom (PFA). We will first discuss examples of the consequences of PFA. We will then present two proper partial orders which are used to force two combinatorial principles which follow from PFA: The P-Ideal Dichotomy (PID) and Todorcevic’s formulation of the Open Coloring Axiom. On one hand, these posets and the proofs of their ...

Journal: :Antimicrobial agents and chemotherapy 1978
S Alenius Z Dinter B Oberg

When applied topically, trisodium phosphonoformate (PFA) displayed activity against established cutaneous herpesvirus infections in guinea pigs similar to that exhibited by the closely related phosphonoacetic acid (PAA); however, unlike PAA, PFA was not locally skin irritating. The therapeutic benefits of topical application of PFA were clearly evident when application was delayed for 48 h afte...

Journal: :Organic letters 1999
C G Ferguson G R Thatcher

[formula: see text] Aminocyclodextrins (ACDs), perfunctionalized at the 6-position with amino groups, bind phosphonoformate (PFA) diesters and accelerate acyl transfer reactions with high efficiency at neutral pH. Aminolysis and esterolysis are accelerated and hydrolysis of PFA diesters is catalyzed by ACDs. PFA diesters have significant antiviral activity. The rapid reactions observed with ACD...

2014
April Galyardt Ilya M. Goldin

We introduce R-PFA, a new model for predicting whether or not a student will answer an item correctly based on the student’s history of practice. The key idea in R-PFA is to represent history as a recentcy-weighted proportion of correct responses. In an evaluation on a dataset from the Assistments tutoring system, we find that R-PFA improves predictive accuracy over other logistic regression mo...

Journal: :European journal of medicinal chemistry 2016
Nyssa Drinkwater Natalie B Vinh Shailesh N Mistry Rebecca S Bamert Chiara Ruggeri John P Holleran Sasdekumar Loganathan Alessandro Paiardini Susan A Charman Andrew K Powell Vicky M Avery Sheena McGowan Peter J Scammells

Malaria remains a global health problem, and though international efforts for treatment and eradication have made some headway, the emergence of drug-resistant parasites threatens this progress. Antimalarial therapeutics acting via novel mechanisms are urgently required. Plasmodium falciparum M1 and M17 are neutral aminopeptidases which are essential for parasite growth and development. Previou...

2015
Chiara Ruggeri Nyssa Drinkwater Komagal Kannan Sivaraman Rebecca S. Bamert Sheena McGowan Alessandro Paiardini Juan Carlos Pizarro

The Plasmodium falciparum PfA-M1 and PfA-M17 metalloaminopeptidases are validated drug targets for the discovery of antimalarial agents. In order to identify dual inhibitors of both proteins, we developed a hierarchical virtual screening approach, followed by in vitro evaluation of the highest scoring hits. Starting from the ZINC database of purchasable compounds, sequential 3D-pharmacophore an...

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