This study investigated biodegradation of dibenzothiophene (DBT) in marsh soil spiked bywhite-rot fungus Phanerochaete chrysosporium. Soil samples were spiked with 100 ppm DBTand incubated at 30°C in a dark chamber for 30 days. Samples were evaluated for pH, Mnperoxidaseactivity, sulfate ion concentration and growth during the tests. Results showedmaximum levels of pH, Mn-peroxidase and sulfate...

waste water from a molasses alcoholic fermentation plant (mww) was treated biologically with phanerochaete chrysosporium. the ability of this white-rot fungus to degrade the dark colored pigments present in mww and the consequent decrease the effluent's color was examined. the optimum concentration of mww for color removal was determined and set at the ratio of 1:10. the color changes during in...

this study investigated biodegradation of dibenzothiophene (dbt) in marsh soil spiked bywhite-rot fungus phanerochaete chrysosporium. soil samples were spiked with 100 ppm dbtand incubated at 30°c in a dark chamber for 30 days. samples were evaluated for ph, mnperoxidaseactivity, sulfate ion concentration and growth during the tests. results showedmaximum levels of ph, mn-peroxidase and sulfate...

The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H(2)O(2)-independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2'-diphenic acid in a reaction that require...

A selective medium was developed that is capable of isolating Phanerochaete chrysosporium from soil. This medium contains 15 ppm of benomyl (15 mug g) and 550 ppm of streptomycin sulfate in 2% malt agar and is held at 39 degrees C after inoculation. P. chrysosporium was isolated from three nonsterile forest soils to which the fungus had been added. These soils contained large microbial populati...

A Phanerochaete chrysosporium cDNA library was constructed in an expression vector that allows expression in both Escherichia coli and Saccharomyces cerevisiae. This expression vector, lambda YES, contains the lacZ promoter for expression in E. coli and the GAL1 promoter for expression in yeast. A number of genes were cloned by complementation of bacterial amino acid auxotrophs. The cDNA encodi...

Of 18 commercially used textile dyes, eight were degraded by the white rot fungus,Phanerochaete chrysosporium, by 40 to 73% based on decrease of colour. Both the lignin-degrading enzyme system ofP. chrysosporium and adsorption to its cell mass were involved in the degradation of the diazo dye, Reactofix Gold Yellow. Degradation was best achieved by adding the dye to the medium and then inoculat...

Heterokaryons made from auxotrophic strains of the lignin-degrading basidiomycete Phanerochaete chrysosporium were induced to fruit. The isolation of wild-type and double-mutant phenotypes from these crosses indicated that genetic recombination had occurred. Cytological studies demonstrated that more than 90% of the basidiospores from the wild-type and auxotrophic strains and from forced hetero...

The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H202independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2'-diphenic acid in a reaction that required Mn(...