Liquid-liquid segmented flows in microchannels have been extensively investigated the context of nanoparticle synthesis. The enhanced mixing slugs results monodispersed particles. Earlier studies focused on Organic-Aqueous Systems (OAS). nanoparticles synthesized presence organic solutions limited applications. An alternative green route for synthesis can be developed using an Aqueous Two-Phase...

phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

Momordica charantia, commonly known as karela or bitter melon, is used as a vegetable in Chinese, Indian subcontinent. However, in Chinese traditional medicine, the plant is usually used as an antidiabetic agent. One of the active components is charantin. Aqueous two-phase systems (ATPS) were used for the purifying saponin because of their low cost. ATPS can reduce amount of organic solvent and...

For a multiproduct microalgal biorefinery, most of the cell components should be extracted and fractionated. This work investigates fractionation lipids from other (pigments, proteins, carbohydrates) using polymers IL solutions in aqueous two-phase systems (ATPS). The poorly migrated to phases ATPS were recovered (97% total fatty acids) third phase (interphase) formed between top bottom phases....

This article provides an analysis on published models used to calculate phase separation in aqueous two-phase systems (ATPS) based Flory-Huggins (FH) theory, terms of problem formulation and mathematical solving algorithm. An integrated algorithm is presented, showing different approaches using the FH theory. The involves estimation interchange energy, calculation compositions. Based experiment...

growing applications of phenylalanine dehydrogenase (phedh) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. with goal of finding a new technique, the extraction and purification of recombinant bacillus badius phedh in polyethylene glycol 6000 (peg-6000) and ammonium sulfate aqueous two-phase systems...

Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems...

In this work, the ability of Na(2)SO(4) and ionic liquids to induce the formation of acidic aqueous two-phase systems (ATPS) is investigated. Ternary phase diagrams, tie lines, and tie-line lengths for several systems were determined and reported at 298 K and atmospheric pressure. It is here shown that among the ionic liquids studied only those containing long alkyl side chains at the ions and/...

−Aqueous two-phase system (ATPS) was applied for extraction bioconversion of xylan by xylanase from Trichoderma viride. Phase diagrams for poly (ethylene glycol) (PEG) and sodium citrate were determined at room temperature. The ATPS composed of 12.99% (w/w) PEG6000 and 12.09% (w/w) sodium citrate was favorable for partition of xylanase and used for extraction bioconversion of xylan. Batch hydro...

An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR...