Previous studies in several laboratories have shown that phosphatidic acid becomes labeled with P3% in cell-free preparations of brain during oxidative phosphorylation (l-3). Kornberg and Pricer (4) found an enzyme in liver which catalyzes the synthesis of phosphatidic acid from a-glycerophosphate and coenzyme A thiol esters of fatty acids. McMurray et al. (2) found this pathway to be present i...

introduction: crude vegetable oils contain some quantities of phospholipids. the highest concentration of phospholipids is usually found in crude soybean oil. the presence of phospholipids in the crude and refined oils create undesirable effects on the quality and quantity of the oil during refining operations or use by the consumer. however, if phospholipids are extracted properly from the oil...

Purified Acetylcholine Receptor (AcChR) from Torpedo has been reconstituted at low (approximately 1:3500) and high (approximately 1:560) protein to phospholipid molar ratios into vesicles containing egg phosphatidylcholine, cholesterol, and different dimyristoyl phospholipids (dimyristoyl phosphatidylcholine, phosphatidylserine, phosphatidylglycerol and phosphatidic acid) as probes to explore t...

Regulators of G-protein signaling (RGS) proteins are critical for attenuating G protein-coupled signaling pathways. The membrane association of RGS4 has been reported to be crucial for its regulatory activity in reconstituted vesicles and physiological roles in vivo. In this study, we report that RGS4 initially binds onto the surface of anionic phospholipid vesicles and subsequently inserts int...

Acetylcholine stimulates the exchange of phosphate in phosphatidic acid in guinea pig brain cortex slices (1) and in cytoplasmic particulate fractions of brain which are undergoing oxidative phosphorylation (2). In this paper the reactions involved in the acetylcholine effect in brain microsomal preparations have been investigated. The data presented here indicate that in brain microsomes phosp...

Incubation of slices of the salt gland of the albatross with acetylcholine, which is the physiological secretogogue for this tissue, led to a 13-fold increase in the rate of incorporation of P(32) into phosphatidic acid and a 3-fold increase in the incorporation of P(32) and inositol-2-H(3) into phosphoinositide. The incorporation of P(32) into phosphatidyl choline and phosphatidyl ethanolamine...

Sphingosine and sphingosine-1-phosphate, metabolites of membrane sphingolipids, have recently been shown to stimulate release of calcium from internal sources and to increase proliferation of quiescent Swiss 3T3 fibroblasts (Zhang, H., Desai, N. N., Olivera, A., Seki, T., Brooker, G., and Spiegel, S. (1991) J. Cell Biol. 114, 155-167). The present study demonstrates that mitogenic concentration...

In the preceding paper evidence was presented for the biosynthesis of lysophosphatidic acid from monoglyceride and adenosine triphosphate (1). Exogenous monoglycende was found to stim.ulate the formation of phosphatidic acid as well as lysophosphatidic acid. This observation suggested that lysophosphatidic acid was being converted to phosphatidic acid and therefore was functioning as an interme...