Phosphatidyl inositol and a cardiolipin-like phospholipid survive the autolysis which is induced in yeast by treating it with toluene, whereas other phospholipids are extensively degraded. Lipid from autolyzed yeast was suspended in isopropanol to extract lecithin and neutral lipid. The insoluble phosphatidyl inositol and cardiolipin were then separated by chromatography on silicic acid.

Phosphatidyl inositol has been isolated from cauliflower inflorescence and soybean. Analysis of the fatty acid distribution showed that unsaturated fatty acids were preferred at position 2, and that saturated fatty acids were preferred at position 1.Mitochondrial preparations from cauliflower inflorescence were capable of synthesizing phosphatidyl inositol from myo-inositol and cytidine diphosp...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

CDP-diglyceride is converted to phosphatidyl inositol by several particulate subcellular fractions of guinea pig brain, with highest specific activity in the microsornal fraction. Optimal conditions with respect to pH, metal ion concentration, and substrate concentrations have been determined. The reaction was stimulated by the addition of bovine serum albumin and by Tween 80. Of several DL-CDP...

Phosphatidyl glycerol, disphosphatidyl glycerol, and phosphatidyl ethanolamine were found in all of the Thiobacillus species studied. T. thioparus possessed only these phospholipids. T. intermedius, T. neapolitanus, and T. thiooxidans contained phosphatidyl-N-monomethylethanolamine, and T. novellus lipids contained phosphatidyl-N-monomethylethanolamine, phosphatidyl-N-N-dimethylethanolamine, an...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

In 1942, Folch and Woolley (1) reported that inositol is a constituent of brain phospholipids. Folch (2) later isolated from brain a lipid fraction containing 2 atoms of phosphorus per mole of inositol. This material, which was incompletely characterized, was termed brain diphosphoinositide. More recent work by Ballou and his co-workers (3, 4) and by Dawson and Dittmer (5) has shown that there ...