The in vitro photodynamic inactivation of a wide variety of tumor cells was studied with various dyes used as sensitizcrs. The capacity of dyes to act as photosensitizers in this system parallels their capacity to sensitize the photoinactivation of many other substrates and correlates with their capacity to undergo photoreduction. Some dyes are able to inactivate tumor cells in the abscnce of l...

The photodynamic action of dyes upon biological systems has been reviewed by Blum (1941). Inactivation of bacteriophage by this process has been studied by Clifton (1931), Perdrau and Todd (1933), Burnet (1933), Morales (1941), and Pacheco and Morales (1941). More recently, Welsh and Adams (1954) and Yamamoto (1956, 1958) investigated the kinetics of photodynamic inactivation of coliphage. In t...

background: biofilm infections are a major challenge in medical practice. bacteria that live in a biofilm phenotype are more resistant to both antimicrobial therapy and host immune responses compared to their planktonic counterparts. so, there is need for new therapeutic strategies to combat these infections. a promising approach [known as photodynamic inactivation (pdi)] to kill bacteria growi...

In this paper we evaluated the efficacy of photodynamic treatment of infected wounds on pig animal model by diffuse reflectance spectrometry. The study was conducted on fifteen wounds contaminated with Staphylococcus aureus bacteria that were incubated for 30 min with methylene blue solution (c = 3.3 x 10 M) and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min. The efficiency of p...

Riboflavin-UVA photodynamic inactivation is a potential treatment alternative in therapy resistant infectious keratitis. The purpose of our study was to determine the impact of riboflavin-UVA photodynamic inactivation on viability, apoptosis and activation of human keratocytes in vitro. Primary human keratocytes were isolated from human corneal buttons and cultured in DMEM/Ham's F12 medium supp...

AIMS To determine the conditions of photodynamic inactivation of vesicular stomatitis virus (VSV) added to pooled coumarin plasma and the effects of the photodynamic treatment on the prothrombin times and international normalised ratio (INR) in a Netherlands national external quality assessment scheme. METHODS Pooled coumarin plasma samples were illuminated with visible light in the presence ...

Toward the objective of developing platform technologies for anti-infective materials based upon photodynamic inactivation, we employed electrospinning to prepare a non-woven textile comprised of polyacrylonitrile nanofibers embedded with a porphyrin-based cationic photosensitizer; termed PAN-Por(+). Photosensitizer loading was determined to be 34.8 nmol/mg material; with thermostability to 300...

Illumination of crayfish giant axons, during internal perfusion with 0.5 mM methylene blue (MB), produces photodynamic effects that include (i) reduction in total sodium conductance, (ii) shifting of the steady-state inactivation curve to the right along the voltage axis, (iii) reduction in the effective valence of steady-state inactivation and, (iv) potentially complete removal of fast inactiv...

Lianrong Wang, Engui Zhao, Sijie Chen and co-workers (article number 2101770) develop a novel membrane-targeting photosensitizer (DTTPB) with aggregation-induced emission characteristics for efficient photodynamic inactivation of human coronaviruses. DTTPB can bind to the envelope coronaviruses sensitize production reactive oxygen species, which effectively inactivate upon white-light irradiati...