Two pilot-scale membrane bioreactors were operated alongside a full-sized activated sludge plant in Tunisia in order to compare specific energy demand and treated water quality. Energy consumption rates were measured for the complete membrane bioreactor systems and for their different components. Specific energy demand was measured for the systems and compared with the activated sludge plant, w...

Hairy roots of Astragalus membranaceus were grown in bioreactors up to 30 l for 20 d. Cultures from a 30 l airlift bioreactor gave 11.5 g l dry wt with 1.4 mg g−1 astragaloside IV, similar to cultures from 250 ml and 1 l flasks, but greater than yields from a 10 l bioreactor (dry wt 9.4 g l−1, astragaloside IV 0.9 mg g−1). Polysaccharide yields were similar amongst the different bioreactors (ra...

During the past 10 years, single-use bioreactors have been well accepted in modern biopharmaceutical production processes targeting high-value products. Up to now, such processes have mainly been small- or medium-scale mammalian cell culture-based seed inoculum, vaccine or antibody productions. However, recently first attempts have been made to modify existing single-use bioreactors for the cul...

Introduction A perfusion-based process was developed to increase the split ratio during the scale-up of CHO-STM cell cultures. Fedbatch cultures were inoculated with cells propagated in either batch or perfusion cultures. All cultures were grown in disposable CellbagTM bioreactors using the WAVE Bioreactor system. Cell concentrations of 4.8 × 10 cells/mL were achieved in the perfusion culture, ...

Introduction A perfusion-based process was developed to increase the split ratio during the scale-up of CHO-STM cell cultures. Fedbatch cultures were inoculated with cells propagated in either batch or perfusion cultures. All cultures were grown in disposable CellbagTM bioreactors using the WAVE Bioreactor system. Cell concentrations of 4.8 × 10 cells/mL were achieved in the perfusion culture, ...

Plants are now gaining widespread acceptance as a general platform for the large-scale production of recombinant proteins. The first plant-derived recombinant pharmaceutical proteins are reaching the final stages of clinical evaluation, and many more are in the development pipeline. Over the past two years, there have been some notable technological advances in this flourishing area of applied ...

Stable integration of a gene into the plant nuclear or chloroplast genome can transform higher plants (e.g. tobacco, potato, tomato, banana) into bioreactors for the production of subunit vaccines for oral or parental administration. This can also be achieved by using recombinant plant viruses as transient expression vectors in infected plants. The use of plant-derived vaccines may overcome som...

Plant expression systems have been developed to produce anti-cancer vaccines. Plants have several advantages as bioreactors for the production of subunit vaccines: they are considered safe, and may be used to produce recombinant proteins at low production cost. However, several technical issues hinder large-scale production of anti-cancer vaccines in plants. The present review covers design str...

All-polyamide composite coated-fabric (APCCF) was used as an alternative material for the construction of textile-bioreactors (TBRs), which are prepared as a replacement of the traditional stainless steel bioreactors (SSBRs) or concrete-based bioreactors. The material characteristics, as well as the fermentation process performance of the APCCF-TBR, was compared with a TBR made using the polyvi...