This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·Lsucrose, 5.5 g·Lagar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·LTDZ, 0.1 mg·LNAA supported the maximal auxil...

In vitro propagation method was developed for obtaining large number of plantlets of Valeriana wallichii DC. (Valerianaceae), an indigenous high value medicinal and aromatic plant species of Indian Himalayan region, using nodes of in vitro grown seedlings. High frequency shoot proliferation was induced in explants cultured on Murashige and Skoog (MS) medium supplemented with different concentra...

The present study was undertaken to investigate the role of banana extract (BE) on synchronous plantlet formation from seeds of Dendrobium lituiflorum Lindl. The seedlings formed on modified Knudson C (KC) medium supplemented with 20% (v/v) BE had significantly high fresh and dry weights. Morphologically uniform plantlets with elongated leaves and well developed roots were formed on KC medium w...

Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture coffea plant in area over 40.000ha South Good quality seedlings have a big demand. Using bioreactor technique the first method resolve lack seedlings. Stem, leaves vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supple...

Thymidine-phosphorylating capacity, mediated by thymidine kinase or a nucleoside phosphotransferase, is closely correlated with DNA synthesis and cell division in a wide variety of bacterial, animal, and plant systems (4, 5, 11, 14, 17, 22). In cells that are not undergoing DNA synthesis and cell division, these enzymes are usually low or undetectable. Conversely, systems undergoing rapid DNA s...

In the last decades the growing awareness of the ecological importance of seagrass meadows has prompted increasing efforts to protect existing beds and restore degraded habitats. An in-depth knowledge of factors acting as major drivers of propagule settlement and recruitment is required in order to understand patterns of seagrass colonization and recovery and to inform appropriate management an...

the present study was undertaken to investigate the role of banana extract (be) on synchronous plantlet formation from seeds of dendrobium lituiflorum lindl. the seedlings formed on modified knudson c (kc) medium supplemented with 20% (v/v) be had significantly high fresh and dry weights. morphologically uniform plantlets with elongated leaves and well developed roots were formed on kc medium w...

An in vitro zygotic embryo germination protocol was developed for apricot (Prunus armeniaca L. “Hacıhaliloğlu”). A hundred percent of germination was obtained when isolated embryos were cultured in vitro, a result alike those reported in the literature with traditional dormancy breaking treatments. In vitro seedling development was optimal in a modified full-strength Murashige and Skoog (1962) ...

Bud break and multiple shoots were induced in apical and axillary meristems derived from one month old seedlings of S. mukorossi on Murashige and Skoog (MS) medium supplemented with benzylamino purine (BAP) 0.4 microM or 0.8 microM alone. A combination of BAP and gibberellic acid (GA3) 0.4 microM and 2.8 microM produced elongated multiple shoots from both types of explants. Excised shoots were ...

Renanthera imschootiana Rolfe is an endangered tropical epiphytic orchid that is threatened with extinction due to over-collection and the loss of suitable habitats. In vitro propagation is a useful way to mass produce plants for re-establishment in the wild and for commercial propagation. Seeds collected 150 days after pollination (DAP) were the optimum stage for in vitro culture. Seed germina...