The arsenical resistance (ars) operon of the conjugative plasmid R773 encodes an ATP-driven anion extrusion pump, conferring bacterial resistance to arsenicals. The operon contains a regulatory gene, arsR, and three structural genes, arsA, arsB, and arsC. The hydrophilic ArsA and ArsC proteins are produced in large amounts, but the hydrophobic ArsB protein, an integral membrane polypeptide, is ...

Plasmid R773, which codes for resistances to arsenate, arsenite, and antimony, was introduced into Escherichia coli strain AN120, a mutant deficient in the H+-translocating ATPase of oxidative phosphorylation. Cultures depleted of endogenous energy reserves were loaded with 74AsO3-4, and arsenate efflux was measured after dilution into medium containing various energy sources and inhibitors. Ra...

conclusions: e. coli herb-resistance plasmid can replicate and be expressed in s. aureus. results: the identified 45 kb herb-resistance plasmid could be transferred from e. coli cp9 isolates to e. coli dh5α. as a consequence e. coli dh5α transconjugant mic increased from 0.0125 g/ml to 0.25 g/ml. the plasmid was easily transferred from e. coli cp9 strain to s. aureus rn450rf with a mean transfe...

background: echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection that occurs by the larval stages of taeniid cestodes of the genus echinococcus . iran is known as endemic region for this infection in the world. vaccination has been considered as a good prevention method for this disease. recombinant vaccines containing eg95 protein, against e. granulosus , has shown a high d...

interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

conclusions based on our findings, p. aerugionsa is a major causative agent of wound infections and amikacin could be considered as a more effective antibiotic for treatment of the burned patients. background nowadays, the treatment of burned patients is difficult because of the high frequency of infection with antibiotic resistance bacteria. objectives this study was conducted to evaluate the ...

Colistin resistance in Acinetobacter spp. is increasing, resulting potentially untreatable nosocomial infections. Plasmid-mediated colistin of particular concern due to its low fitness cost and potential transferability other bacterial strains species. This study investigated the mechanism a clinical nosocomialis isolate from Cape Town, South Africa. A colistin-resistant A. was identified blood...

the biodegradation of secondary amines is particularly important due to their propensity for conversion either chemically or microbiologically to n-nitrosamines which are potent carcinogens. in this research, a weak gram-positive organism was isolated from river and identified as mycobacterium. this mycobacterium grows slowly and effectively utilizes piperazine as the sole source of organic, ca...