BACKGROUND Our previous studies have demonstrated that the levels of 5-hydroxytryptamine (5-HT) and 5-HT 2A receptor (5-HT2AR) in serum and platelet were associated with depression and myocardial infarction (MI), and pretreatment with ginseng fruit saponins (GFS) before MI and depression had an effect on the 5-HT system. In this study, the effects of GFS on the 5-HT system in the Sprague-Dawley...

Efficient xenofree expansion methods to replace fetal bovine serum (FBS)-based culture methods are strongly encouraged by the regulators and are needed to facilitate the adoption of mesenchymal stromal cell (MSC)-based therapies. In the current study we established a clinically-compliant and reproducible animal serum-free culture protocol for bone marrow-(BM-) MSCs based on an optimized platele...

Human platelet lysate was shown to contain growth-promoting activity for four well-established malignant cell lines. Platelet lysate was able to support their cell proliferation without plasma or serum, indicating that platelets contained "survival factor" as well as mitogenic factor for these tumor cells. The growth-promoting activity was nondialyzable, heat stable up to 56 degrees, and partia...

1. Under optimal ionic conditions (4 mM-MnCl2) the specific activity of guanylate cyclase in fresh platelet lysates was about 10nmol of cyclic GMP formed/20 min per mg of protein at 30 degrees C. Activity was 15% of optimum with 10mM-MgCl2 and negligible with 4mM-CaCl2. Synergism between MnCl2 and MgCl2 or CaCl2 was observed when [MnCl2] less than or equal to [GPT]. 2. Lower than optimal specif...

Abstract Purpose At present, autologous platelet-derived therapies such as platelet-rich plasma is widely used in the clinic, but allogeneic and xenogeneic are currently research stage, mainly due to their biocompatibility vivo. Platelet lysates depleted of antigens blood cells a potential solution for or applications. can successfully promote growth differentiation vitro, vivo data not yet ava...

background: the autoimmune disease known as idiopathic (immune thrombocytopenic purpura thrombocytopenic purpura (itp) is clinically defined by a low numbers of platelets in the circulation blood. this study aimed to isolate autoantibodies made against the platelet glycoproteins using platelets from healthy volunteers, to determine their specificity and further elucidate their effects on platel...

Background: Recent improvement in treatments of cancer has significantly increased survival rates of cancer patients. Unfortunately these treatments such as chemotherapy and radiotherapy can sever damage to the ovaries. Ovarian cryopreservation prior to chemo/ radiotherapy and auto-transplantation post-treatment can restore fertility to cancer patients. Hypoxia in the transplant from delayed re...

Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on ...