Poly(3-hydroxybutyrate) (PHB) has been proposed to be a potential candidate used as biomaterial. However, its poor processability, high brittleness and rigidity have limited applicability. Transesterification reactions with poly(e-caprolactone) (PCL), one of the most promising biomedical materials, emerge an attractive alternative improve mechanical properties. In this work, poly(3-hydroxybutyr...

The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimu...

The equilibrium adsorption of bovine serum albumin (BSA) on poly-3-hydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) surfaces was studied at different solution concentrations. data were fitted to the Langmuir Freundlich models. protein behaviour further investigated by analysing conformation surface adsorbed BSA using Fourier transform infrared (FTIR) spectroscopy wi...

plackett-burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of methylobacterium extorquens dsmz 1340. among the studied variables, nitrogen and phosphorus sources, mgso4 and most of the trace elements were found to be significant variables for phb production from methan...

Original Article Alcaligeneseutrophus H16, E. coli DH5α, operon phbCAB, Poly 3-Hydroxybutyrate (PHB).

BACKGROUND Poly-beta-hydroxybutyrate (PHB) mobilization in bacteria has been proposed as a mechanism that can benefit their host for survival under stress conditions. Here we reported for the first time that a stress-induced system enabled E. coli, a non-PHB producer, to mobilize PHB in vivo by mimicking natural PHB accumulation bacteria. RESULTS The successful expression of PHB biosynthesis ...

the taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(phb) production by ralstonia eutropha. the optimum condition was obtained as: fructose concentration, 15 g/l; c/n ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° c; seed age, 15 h. at optimum condition the yield of phb pr...

Rhodospirillum rubrum possesses a putative intracellular poly(3-hydroxybutyrate) (PHB) depolymerase system consisting of a soluble PHB depolymerase, a heat-stable activator, and a 3-hydroxybutyrate dimer hydrolase (J. M. Merrick and M. Doudoroff, J. Bacteriol. 88:60-71, 1964). In this study we reinvestigated the soluble R. rubrum PHB depolymerase (PhaZ1). It turned out that PhaZ1 is a novel typ...

Cyanobacteria have many unexploited potential for natural products with a huge variability in structure and biological activity. Under stress conditions they are reported to produce biopolymers like poly-β-hydroxybutyrate (PHB), which can be produced intracellularly.Cyanobacteria are capable of synthesizing small amount of poly-β-hydroxybutyrate (PHB) under nitrogen and phosphorous starvation c...

Biopolymers are a promising alternative to petroleum-based plastic raw materials. They bio-based, non-toxic and degradable under environmental conditions. In addition the homopolymer poly(3-hydroxybutyrate) (PHB), there number of co-polymers that have broad range applications easier process in comparison PHB. The most prominent representative from this group bio-copolymers is poly(3-hydroxybuty...