Objectives We aimed to develop a sustained release system for an anti-TNFa drug in treatment of chronic inflammatory arthritis. A novel form of intra-articularly injectable etanercept (ETN) loaded poly(ε-caprolactone) (PCL) or methoxy poly(ethylene glycol)-poly(ε-caprolactone)methoxy poly(ethylene glycol) (MPEG-PCL-MPEG) microspheres (patent pending) were prepared to provide long term controlle...

Marrow-derived osteoblasts were cultured on poly(propylene fumarate-co-ethylene glycol) (P(PF-co-EG)) based hydrogels modified in bulk with a covalently linked RGDS model peptide. A poly(ethylene glycol) spacer arm was utilized to covalently link the peptide to the hydrogel. Three P(PF-co-EG) block copolymers were synthesized with varying poly(ethylene glycol) block lengths relative to poly(eth...

A short, monodisperse additive (octa(ethylene glycol) monophenyl ether) functions to suppress aggregation of thermally and chemically denatured lysozyme. Control studies with shorter and non-amphiphilic derivatives revealed that the amphiphilic structure is essential, and octa(ethylene glycol) is nearly the minimum chain length for amphiphilic poly(ethylene glycol)s to stabilize proteins.

Erythrocytes from multiple sclerosis patients differ significantly (p less than 0.005) from those from controls with regard to hydrophobic affinity partition in two-polymer aqueous-phase systems containing dextran, poly(ethylene glycol) and poly (ethylene glycol)-fatty acid esters. The most likely source of the abnormality is the cell membrane.

We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single alpha-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit...

Polymer-peptide conjugates are a promising class of compounds, where polymers can be used to overcome some of the limitations associated with peptides intended for therapeutic and/or diagnostic applications. Linear polymers such as poly(ethylene glycol) can be conjugated through terminal moieties and have therefore limited loading capacities. In this research, functionalised linear poly(ethylen...

Poly(ethylene glycol)-based nanoparticles have received significant attention in the field of biomedicine. When they are copolymerized with pHor temperature-sensitive comonomers, their small size allows them to respond very quickly to changes in the environment, including changes in the pH, ionic strength, and temperature. In addition, the high surface-to-volume ratio makes them highly function...

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated...