Pepsin activity was demonstrated in circulating immune complexes, isolated from sera of 90 diabetic patients, which included 30 type 1, 60 type 2 diabetic patients and 40 control subjects, by the polyethylene glycol method. Pepsin activity was estimated by the proteolytic assay, using acidified haemoglobin as substrate. The mean polyethylene glycol indices in type 1 and type 2 diabetic patients...

We evaluated 10 microfilters for their ability to filter diluted sera without removing immunoglobulins (Ig) G, A, and M, albumin, and transferrin. In general, filters containing cellulose nitrate remove IgG from solution, the amount adsorbed being proportional to the IgG concentration in the solution. With some sera we noted IgA and IgM adsorption to cellulose-nitrate-containing filters, but th...

Background: There are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. There are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. Ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. This resin was used to isolate highly purified insulin receptor from human placental tissue by a four step process involving (i) preparation of a crude membrane fraction, (ii) solubilization with Triton X-100, (iii) wheat germ aggluti...

silymarin, an extract from silybum marianum, has been shown to have antioxidant properties. however, there is no scientific report on wound healing activity of the silymarin. the purpose of this study was to evaluate the effect of topical administration of silymarin on excision wound healing in rats. excision wounds were made on the back of rats. rats were divided into three groups, as control,...

BACKGROUND AND PURPOSE The purpose of this study was to determine the distribution of polyethylene glycol-conjugated superoxide dismutase in the brain, cerebrospinal fluid, and various organs. METHODS Distribution of iodine-125-labeled polyethylene glycol-conjugated superoxide dismutase was determined in three groups of male Sprague-Dawley rats: a normotensive sham control group (n = 9) and g...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

emptied and washed, and the 24h efflux of inulin was determined; it is plotted against the final swelling ratio from the experiment in which the sac was stretched. An unstretched control is also included. In a similar unstretched sac, the half-time of raffinose escape was I4Omin. Clearly inulin escapes faster than the polyethylene glycol of comparable molecular weight at all porosities, but at ...

BACKGROUND AND PURPOSE Transport of urea across the blood-brain barrier is increased during postischemic cerebral reperfusion in the piglet. Ischemia/reperfusion also has been observed to increase apparent superoxide anion generation on the surface of the brain. The present study was designed to address the hypothesis that the increased transfer of urea into the brain after ischemia/reperfusion...

THE USE of polyethylene glycol (PEG) in preservation solutions has been associated with a decreased incidence of rejection in clinical and experimental transplantation. Using the isolated perfused rat liver model, we found that PEG, when added to phosphate-buffered sucrose solution (PBSL), provided improved liver preservation. A direct comparison of three different molecular configurations of P...