?????????????coronavirus disease 2019? COVID?19?????????????????????acute kidney injury? AKI????????????????????????????????????????????????????????????????????10?????????????????????????????????????????????COVID?19????????????????????????????????severe acute respiratory syndrome coronavirus 2? SARS?CoV?2????????????????????????????????????????9???????????????????????????reverse transcription p...

The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

background aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). objectives this study aimed to evaluate the frequency of aseptic meningitis caused by herpesviruses, namely herpes simplex types i and ii (hsv-1, hsv-2), epstein-barr virus (ebv), cytomegalovirus (...

conclusions in the present study, the highest prevalence was for st5, which is so important for epidemiology and risk of human infection. the report related to st3 in cattle as a subtype of human showed mutual infection between human and cattle. another important point in this study was the st6 report. finally, it seems that gathering epidemiological data is needed for a better understanding of...

background toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. the parasite is able to infect all warm-blooded hosts including human.the infection occures via consumption of food or water containing oocytes, eatingundercooked meats containing tissue cysts, and placenta. undercooked meat consumption is one of the most important ways of toxoplasma transm...

conclusions using the pcr-rflp method, three distinctive h. pylori caga genotypes were detected in antral biopsies. genotype i, which was predominant among the isolates, was significantly associated with gastritis. however, the data showed that caga genotype iii may play a role in duodenitis and duodenal ulcers in patients infected with h. pylori. patients and methods a total of 161 gastric bio...

background human t-lymphotropic virus type i (htlv-i) is one of the health threatening problems in endemic areas and can be transmitted by different routes such as blood transfusion. in order to correct chronic anemia in thalassemia subjects, they may need to get blood units per month. thus, they are at risk of infection with blood-borne viruses such as htlv-i. objectives in the present study, ...