In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of ...

Spinach (Spinacia oleracea L.) is an economically important leafy vegetable crop in many countries. This is the first case study of using SRAP markers to analyze genetic diversity of Iranian spinach accessions. Eight SRAP primer combinations generated 88 scorable bands ranging from 50 to 1000 bp, among which 73 were polymorphic, with an average of 82.9 polymorphic bands per primer combination a...

Finger millet is an important cereal crop in eastern Africa and southern India with excellent grain storage quality and unique ability to thrive in extreme environmental conditions. Since negligible attention has been paid to improving this crop to date, the current study used Next Generation Sequencing (NGS) technologies to develop both Simple Sequence Repeat (SSR) and Single Nucleotide Polymo...

For pollination purpose and apiary by-products production Apis cerana indica is highly demanded honey bee in India. Honey a polymorphic having three casts queen, workers drones. In queen undergoes multiple mating with drones to gather genetic diversity colony. Genomic DNA sample of adult drone worker bees was used study polymorphism by Random amplified (RAPD) markers. Ten Primers each containin...

the japanese and english white quails are widespread strains and belongs to the galliformes order, phasianidae family, coturnix genus  and japonica species. these birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. in the current study, the genetic variation of japanese and english white  quail ...

Genetic diversity of 12 lemon balm ecotypes was evaluated using agronomic traits as well as ISSR and RAPD markers. Results of analysis of variance showed high diversity among the studied ecotypes. Cluster analysis using UPGMA method grouped the ecotypes into three different groups based on the agronomic traits. Twelve ISSR primers created 106 polymorphic bands among the studied ecotypes. An ISS...

Microsatellites, tandem repeats of short nucleotide (1-6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within genome, ease of genotyping through polymerase chain reaction (PCR), selectively neutral, co-dominant and multi allelic nature. Six microsatellites, three polymorphic and three monomorphic, have been characterized for the first t...

The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). Within the nine polymorphic loci, allele frequencies, number of effective alleles (Ne), heterozygosity (He), polymorphism information content (PIC), genetic identity (I) and Nei’s standard genetic ...

We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, consisting of 7 random amplified polymorphic DNA and 17 inter-simple sequence repeat markers were used to estimate the genetic diversity of 20 Anth...