von Willebrand factor/ristocetin (vWF/R) induces GPIb-dependent platelet agglutination and activation of αIIbβ3 integrin, which also binds vWF. These conditions make it difficult to investigate GPIb-specific signaling pathways in washed platelets. Here, we investigated the specific mechanisms of GPIb signaling using echicetin-coated polystyrene beads, which specifically activate GPIb. We compar...

We evaluated the effectiveness with which polystyrene beads separate serum from blood. Paired blood samples were analyzed for 23 different serum constituents. One specimen of each pair was processed by the conventional separation method, the other by a single centrifugation with the polystyrene beads. Differences between the assay means were evaluated by the z-test, and the data pairs were comp...

We report a study on particle diffusion in membranes formed from polystyrene-block-poly(2-(dimethylamino)ethyl methacrylate) (PS-b-PDMAEMA) diblock copolymers. The membranes were investigated by scanning electron microscopy and by single-particle tracking employing carboxy-functionalized polystyrene beads loaded with a fluorophore as spectroscopic probes. From the diffusion trajectories we extr...

Macrophages treated with polystyrene beads of different sizes show adaptive responses but no inflammatory responses.

Monodispersed polystyrene beads incorporated with Fe(3) O(4) nanoparticles are prepared via dispersion polymerization. The resultant magnetic beads present well-defined composite structures, excellent colloidal stability, and strong magnetic response. The formation mechanism for the monodispersed composite beads, incorporated with preformed Fe(3) O(4) nanocrystals, was investigated. The potenti...

Novel quantum-dot-tagged photonic crystal beads were fabricated for multiplex detection of tumor markers via self-assembly of quantum dot-embedded polystyrene nanospheres into photonic crystal beads through a microfluidic device.

Problem statement: Polystyrene failed to provide any reactive functionality of surface hydrophilicity that is capable of binding proteins. It is known that polystyrene must be chemically modified to make its surface amenable to covalent crosslinking with protein. Approach: The aim of this study was to investigate the effects of UV/ozone treatment on gelatin coating. The surfaces of microsize po...

A novel approach for light-dependent covalent immobilisation of synthetic DNA oligomers to amino-coated paramagnetic beads is described. A hetero-bifunctional photo-reactive cross-linking chemical, 4-nitrophenyl 3-diazopyruvate, is applied to attach 5' amino-modified DNA to both silica and polystyrene paramagnetic beads. The coupling yields are comparable with similar methods in which no photo-...

Our aim was to elaborate a novel method for fully controllable large-scale nanopatterning. We investigated the influence of the surface topology, i.e., a pre-pattern of hydrogen silsesquioxane (HSQ) posts, on the self-organization of polystyrene beads (PS) dispersed over a large surface. Depending on the post size and spacing, long-range ordering of self-organized polystyrene beads is observed ...

Electron microscopic studies confirm and extend the conclusions derived previously from a quantitative biochemical study of the phagocytosis of polystyrene and polyvinyltoluene latex beads by Acanthamoeba (1). Latex beads 1.305, 1.90, and 2.68 micro in diameter are ingested individually, with each bead tightly surrounded by a membrane derived from the plasma membrane. Latex beads 0.557, 0.264, ...