PURPOSE To study the usefulness of simultaneous combination culture with egg-based Ogawa medium (1 slant) and MGIT (Mycobacterium growth indicator tube/Becton-Dickinson) for detection of M. tuberculosis from sputum in sputum-culture proven pulmonary tuberculosis patients. OBJECT AND METHOD Retrospective study of sputum-culture-positive pulmonary tuberculosis cases in our hospital (with tuberc...

A total of 96 sputum specimens from patients with suspected or known mycobacterial and nonmycobacterial pulmonary infections were analyzed by gas chromatography-mass spectrometry for the presence of 2-eicosanol. This secondary alcohol was detected in all of the 25 sputum specimens culture positive for Mycobacterium tuberculosis, in 7 of the 9 sputum specimens culture positive for M. avium compl...

Aspergillus fumigatus sensitization and culture in asthma are associated with disease severity and lung function impairment, but their relationship with airway inflammation is poorly understood. We investigated the profile of 24 sputum inflammatory mediators in A. fumigatus culture-positive or-negative moderate-to-severe asthmatics. Fifty-two subjects were recruited from a single center. A. fum...

At present, further investigations are needed in patients with suspected pulmonary tuberculosis (TB) and either negative sputum smear or without sputum. The aim of the present study was to analyse the yield of bronchoalveolar lavage fluid (BALF) smear and PCR in patients with confirmed pulmonary TB. Patients with a positive culture for Mycobacterium tuberculosis complex in sputum or BALF were a...

The aim of this study was to assess the value of examining multiple sputum specimens in the diagnosis of pulmonary tuberculosis (PTB). We analyzed sputum smear and culture results of patients diagnosed with culture-proven PTB during 2002. In 1027 patients, the diagnosis was established by detection of Mycobacterium tuberculosis bacilli in sputum samples. The number of sputum specimens submitted...

BACKGROUND The diagnosis of active pulmonary tuberculosis (TB) remains a challenge in clinic, especially for sputum negative pulmonary TB. Bronchoalveolar lavage fluid (BALF) has higher sensitivity than sputum for detection of Mycobacterium tuberculosis (Mtb). However, bronchoscopy is invasive and costly, and not suitable for all patients. In order to make TB patients get more benefit from BALF...

BACKGROUND The impact of tuberculosis (TB) is exacerbated in Africa because of the human immunodeficiency virus (HIV) pandemic. Pulmonary tuberculosis (PTB) diagnosis is difficult in HIV-infected patients and negative sputum results are more common which leads to diagnostic delay and increases morbidity and mortality. Extra-pulmonary samples such as stool may be easier to obtain and our approac...

OBJECTIVE To compare the diagnostic yield of AFB positivity with sputum induction to spontaneous sputum examination in suspected cases of pulmonary tuberculosis. STUDY DESIGN Comparative study. PLACE AND DURATION OF STUDY Military Hospital, Rawalpindi, from January to December 2006. METHODOLOGY Sputum specimens were collected by both techniques from 164 patients with clinical and radiolog...

This prospective study was conducted in 6 hospitals in Edmonton, Canada to determine the factors associated with obtaining sputum for culture and the effect of sputum culture on the management of patients with community-acquired pneumonia (CAP). Participants were 1362 adults who were hospitalized with CAP. Sputum was obtained from 539 (39.6%) patients, of which 507 (94.1%) were good quality, ac...

BACKGROUND Previous studies suggest that bronchoscopy and a single induced sputum sample are equally effective for diagnosing pulmonary tuberculosis. METHODS In a prospective study of subjects with possibly active pulmonary tuberculosis, the diagnostic yield of three induced sputum tests was compared with that of bronchoscopy. Subjects either produced no sputum or (acid fast) smear negative s...