purpose: to assess the short-term effect of mitomycin-c (mmc) 0.02% on endothelial cell density and morphology after photorefractive keratectomy (prk) in patients with moderate myopia. methods: forty-two eyes of 21 participants with moderate myopia (range, -4.0 to -8.0 d) underwent prk with mmc 0.02% for 40 seconds. specular microscopy was performed preoperatively and repeated 6 months after su...

OBJECTIVE To evaluate Descemet membrane endothelial keratoplasty for management of corneal endothelial disorders. METHODS Descemet membrane endothelial keratoplasty was performed in 200 patients with Fuchs endothelial dystrophy or bullous keratopathy. Best-corrected visual acuity, subjective and objective refractive outcome and stability, and endothelial cell density were evaluated at 1, 3, a...

Purpose To determine the factors that influence the endothelial cell density (ECD) of donor grafts after Descemet stripping automated endothelial keratoplasty (DSAEK). Methods This retrospective, interventional case series comprised 77 eyes of 64 patients who underwent DSAEK. Confocal microscopy was performed at the final follow-up examination to evaluate the endothelial cell count, cell morp...

PURPOSE To compare 2-year clinical outcomes of Descemet's stripping automated endothelial keratoplasty (DSAEK) and penetrating keratoplasty (PK) in patients with bullous keratopathy. METHODS A retrospective chart review was performed to obtain 2 years of follow-up data of DSAEK or PK at a single center from March 2009 to September 2012. The study comprised 15 eyes of DSAEK and 11 eyes of PK. ...

PURPOSE To evaluate retrospectively the corneal endothelium in 15 eyes which showed clear cornea for 20 years or longer after penetrating keratoplasty. METHODS The corneal endothelium was investigated in 15 eyes. The causative lesion had been keratoconus in 10 eyes and herpetic keratitis in 5 eyes. At the time of surgery, the patients were aged from 6 to 45 years, average 25.3 +/- 10.4 years....

IMPORTANCE Endothelial morphometric measures have potential value in predicting graft failure after penetrating keratoplasty. OBJECTIVE To determine whether preoperative and/or postoperative central morphometric measures (endothelial cell density [ECD], coefficient of variation [CV], and percentage of hexagonality [HEX]) and their postoperative changes are predictive of graft failure caused b...

objective: in this investigation murine embryonic stem (es) cells were differentiated into endothelial cells. materials and methods: murine es cells (cce cell line) exposed to alpha-mem medium containing 10% fbs for 4 days. then obtained flk-1 (flk-1: vascular endothelial growth factor receptor 2) positive cells were cultuted in endothelial growth medium-2 (egm-2) until the last day of experime...

BACKGROUND Although inhibition of neointimal hyperplasia by cell cycle gene blockade therapy results in improved endothelial cell function in experimental vein grafts, little is known either about endothelial healing immediately after vein grafting or about the effect of this therapy on the healing process. METHODS AND RESULTS Scanning electron microscopy demonstrated an immediate decrease in...

Here we report a case of endothelial keratoplasty with infant donor tissue obtained after brain death. A 52-year-old man with endothelial dysfunction of unknown cause in the right eye underwent non-Descemet stripping automated endothelial keratoplasty (nDSAEK) with tissue from an infant donor (2 years). Intraoperative and postoperative complications were recorded. Best corrected visual acuity a...