ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene ...

We have previously isolated and sequenced a portion of the structural gene for tetanus toxin from C.tetani CN3911 (1). Overlapping clones encoding the entire gene have now been isolated and the full sequence is reported below. Our designation of the start of translation is based on amino terminal analysis (2) and partial ami no acid sequence of the toxin (3). The methionine residue before the p...

abstract various resistances to salinity are due to plants genetically variations, and selection on the basis of one factor will not be a suitable criterion in measurement of resistance to salinity, therefore in choosing resistant varieties, it is necessary to notice to a set of resistant indexes to salinity, creating changes in physiology, anatomy and morphology of plant species. this research...

The PSI/TM-Coffee web server performs multiple sequence alignment (MSA) of proteins by combining homology extension with a consistency based alignment approach. Homology extension is performed with Position Specific Iterative (PSI) BLAST searches against a choice of redundant and non-redundant databases. The main novelty of this server is to allow databases of reduced complexity to rapidly perf...

After vacuum distillation and liquid-liquid extraction, the volatile fractions of dark chocolates were analyzed by gas chromatography-olfactometry and gas chromatography-mass spectrometry. Aroma extract dilution analysis revealed the presence of 33 potent odorants in the neutral/basic fraction. Three of these had a strong chocolate flavor: 2-methylpropanal, 2-methylbutanal, and 3-methylbutanal....