Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...

This paper describes an evaluation of an online Student Induction and Support Package used to prepare both face-to-face and fully online students for learning online. It provides an overview of two components of the Package, the Primer and Survival Guide. It details the type and the nature of the student feedback requested via an online questionnaire and presents the results obtained. Key findi...

Before performing polymerase chain reactions (PCR), a feasible primer set is required. Many primer design methods have been proposed for design a feasible primer set. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specifi...

Single-nucleotide polymorphism (SNP) genotyping can be carried out by annealing an oligonucleotide primer directly adjacent to the polymorphism and carrying out a single base extension using a polymerase reaction with labeled dideoxynucleotide triphosphates. This can be multiplexed by attaching a unique tag at the 5'-end of each oligonucleotide primer and binding the corresponding antitag to a ...