Promoter methylation is one of the main epigenetic mechanisms that lead to the inactivation of tumor suppressor genes during carcinogenesis. Due to the reversible nature of DNA methylation, many studies have been performed to correct theses epigenetic defects by inhibiting DNA methyltransferases (DNMTs). In this case novel therapeutics especially siRNA oligonucleotides have been used to specifi...

Sclerotinia sclerotiorum is a phytopathogenic fungus which causes serious yield losses in canola. A pathogen inducible-promoter can facilitate the production of Sclerotinia-resistant transgenic canola plants. Inthis study, the “gain of function approach” was adopted for the construction of a pathogen-inducible promoter.The synthetic promoter technique was used, which involved the in...

many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...

Background: Epigenetic modifications, such as methylation can occur in multiple myeloma. SMG1is an important gene involved in cell growth which defect in methylation of its promoter leads to reduction of cell apoptosis and uncontrolled proliferation. In this study, we identified the methylation status of the SMG1 gene promoter in patients with multiple myeloma. Methods: Methylation status of S...

There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...

objective: in an attempt to develop safer and more effective gene therapy approaches as a realistic treatment for various forms of cancer, researchers are increasingly using tumor-specific promoters (tsp) to drive the expression of the gene of interest and eradicate cancer cells. in this study, for the first time we introduce the oct-4 promoter as a cancer-specific promoter with a high efficacy...

background: recently, we have shown that peroxisomal protein expression was induced upon retinoic acid treatment in mouse embryonic stem cells during the process of neurogenesis. thus, characterization of the respective promoter could elucidate the molecular aspects of transcriptional regulation of this gene. methods: using the conventional software programs for promoter prediction, a putative ...