background: fasciola hepatica is one of the most important helminthes parasites and triclabendazole (tcbz) is routinely used for treatment of infected people and animals. secreted protease enzymes by the f. hepatica plays a critical role in the inva-sion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. the aim of study was to determine the pro...

screening and identification of moderately halophilic protease producing bacteria from different regions of maharloo, a hypersaline lake in the southern area of iran, were the objectives of this study. in the preliminary screening, 16 isolates exhibited proteolytic activity on saline skim milk agar. all isolates were able to grow comfortably in the media containing 7-15% of salt. protease activ...

Kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, Salinivibrio sp. strain MS-7, and maximum growth and protease activity was achieved after 48 hours at 30°C and 180 rpm. To determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and  maximum production of...

entomopathogenic fungi produce a variety of degrading enzymes, including proteases, chitinases and lipases, to facilitate their entry through the massive barriers of insect cuticle. isolates of the entomopathogenic fungi vary considerably in their proteolytic activity and virulence. the proteolytic activity of different isolates has been hypothesized to reflect their virulence toward the host. ...

Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi.<br...

background: hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albendazole (abz) and mebendazole (mbz). the purpose of this study was comparing the effects of abz and mbz on glutathione-s-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst. methods: the culture supernatants containing the parasite excretory...

the aim of the present work was to purify cysteine protease inhibitors from rapeseed (brassica napus l.), with potential activity on digestive protease of colorado potato beetle (cpb), leptinotarsa decemlineata (say). ammonium sulfate precipitated proteinaceous fractions; 30, 50, 70, and 100% showed 39.07, 57.03, 51.47, and 22.44% inhibition on the fourth instar larval gut general protease acti...

      Screening and identification of moderately halophilic protease producing bacteria from different regions of Maharloo, a hypersaline lake in the southern area of Iran, were the objectives of this study. In the preliminary screening, 16 isolates exhibited proteolytic activity on saline skim milk agar. All isolates were able to grow comfortably in the media containing 7-15% of salt. Protease...

background: one of the most important producers of high quality industrial enzymes is the gram-positive bacterium, bacillus subtilis (b. subtilis). one major limitation that hinders the wide application of b. subtilis is the secretion of high levels of extracellular proteases which degrade the secreted foreign proteins. in this study, homologus recombination technique was used to knock out its ...

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...