Aims: Extract proteins with protease inhibitor (PI) activity from fresh organs of Cajanus cajan, using aqueous systems; study the melanoma secreted proteases; investigate inhibitory effect C. cajan extracts on and evaluated extract, most activity, cell line (SK-MEL-28) viability.
 Material Methods: Extracts leaves, stems, roots were prepared different systems. Protein content was by Bradfo...

The plant apoplast is a harsh environment in which hydrolytic enzymes, especially proteases, accumulate during pathogen infection. However, the defense functions of most apoplastic proteases remain largely elusive. We show here that newly identified small cysteine-rich secreted protein PC2 from potato late blight Phytophthora infestans induces immunity Solanum plants only after cleavage by subt...

Viral infection imposes a major threat to human health. To develop new potent antiviral agents, Hederagenin (HE), known inhibitor of HIV-1 and HCV NS3/4A proteases, was used as starting material synthesize 4 types HE derivatives, HE-3,23-diacyl, HES-3,23-diacyl, HES-3-acyl, HES-3-oxo-23-acyl. We evaluated the in vitro inhibitory activities derivatives against proteases. (3 β,23)-Di- O-diglutary...

Fish epidermal mucus contains innate immune components, secreted by globlet cells that provide the primary defence against different pathogenic microbes and act as a barrier between fish and its immediate niche. The major function of mucus includes entrapment and sloughing of microbes. The mucus also contains many factors such as antimicrobial peptides (AMPs), lysozymes, lectins, proteases, etc...

proteases play a key role in protein digestion in ticks and other haematophagous insects. our understanding of blood meal digestion in digestive system of ticks can be very useful for better understanding of basic rules for control of ticks. cells of the midgut endocytose blood components. blood proteins uptake by midgut cells, suggesting the presence of proteases in the midgut cells. in this s...

Coronavirus entry is mediated by the spike protein that binds receptor and mediates fusion after cleavage host proteases. The proteases mediate differ between cell lines, it currently unclear which are relevant in vivo. A remarkable feature of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presence a multibasic site (MBCS), absent SARS-CoV spike. Here, we report SARS-CoV-2 MBCS in...