objective: the aim of this experiment was to determine chemical composition, protein quality of corn gluten meal (cgm) and its effect on growth performance of broiler chicks. methods: chemical composition and nitrogen corrected true metabolizable energy of cgm was determined by standard and precision-fed cockerel assay methods.  for evaluating of protein quality, 90 male broiler chicks in a com...

Background: The B-cell defect in X-linked agammaglobulinemia (XLA) is caused by mutations in the gene for Bruton's tyrosine kinase (BTK). BTK mutations result in deficient expression of BTK protein in peripheral blood monocytes. Methods: Using the anti-BTK monoclonal antibody (48-2H), a flow cytometric analysis of intra cytoplasmic BTK protein expression in monocytes was performed to identify I...

background: the b-cell defect in x-linked agammaglobulinemia (xla) is caused by mutations in the gene for bruton's tyrosine kinase (btk). btk mutations result in deficient expression of btk protein in peripheral blood monocytes. methods: using the anti-btk monoclonal antibody (48-2h), a flow cytometric analysis of intra cytoplasmic btk protein expression in monocytes was performed to ident...

Protein-protein interactions (PPI) are involved in a myriad of cellular processes, and their deregulation can lead to many diseases. One such process is protein ubiquitination that requires an orchestrated action three key enzymes add ubiquitin moieties substrate proteins. Importantly, this reversible through deubiquitinating enzymes. Both deubiquitination require PPIs once classified be utiliz...

background: ionizing radiation causes deleterious effects on living system mainly due to oxidative damages of macromolecules and protein is the major target due to its abundance. the aim of this study was to investigate the effects of ionizing radiation induced changes in the molecular properties of bovine serum albumin (bsa) its secondary and tertiary structures, degradation, cross linking and...

Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...

Background: Ionizing radiation causes deleterious effects on living system mainly due to oxidative damages of macromolecules and protein is the major target due to its abundance. The aim of this study was to investigate the effects of ionizing radiation induced changes in the molecular properties of bovine serum albumin (BSA) its secondary and tertiary structures, degradation, cross linking and...

Here, we developed a rapid and quantitative one-step severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen assay based on protein-induced fluorescence enhancement (PIFE) of nucleocapsid (N) protein DNA aptamer.