this study was conducted to evaluate the cytoprotection of various extracts and bioactive compounds found in pistacia vera againts cytotoxicity, ros formation, lipid peroxidation, protein carbonylation , mitochondrial and lysosomal membrane damages in cell toxicity models of diabetes related carbonyl (glyoxal) and oxidative stress (hydroperoxide). methanol, water and ethyl acetate were used to ...

Protein carbonyls are widely analysed as a measure of protein oxidation. Several different methods exist for their determination. A previous study had described orders of magnitude variance that existed when protein carbonyls were analysed in a single laboratory by ELISA using different commercial kits. We have further explored the potential causes of variance in carbonyl analysis in a ring stu...

An n = π* interaction between neighboring carbonyl groups has been postulated to stabilize protein structures. Such an interaction would affect the (13)C chemical shielding of the carbonyl groups, whose paramagnetic component is dominated by n = π* and π = π* excitations. Model compound calculations indicate that both the interaction energetics and the chemical shielding of the carbonyl group a...

Introduction: Diabetes is chronic disease which characterised by defect in insulin secretion and action leading to hyperglycaemia. Aim: To detect protein carbonyl as oxidative stress marker Type 2 Mellitus (T2DM). Materials Methods: The present cross-sectional study was conducted on 100 subjects (aged 40 70 years) out of 50 were T2DM normal individuals. Serum tested for Fasting Blood Sugar (FBS...

Objectives. The aim of the present investigation was to study the activity of glucose-6-phosphate dehydrogenase (G6PD) and correlate its activity to protein oxidation markers in type 2 diabetic patients under poor glycemic control. Methods. G6PD activity, protein carbonyl group concentration, and total thiol group content were measured in blood samples of 40 patients with type 2 diabetes mellit...

A dysregulation of the redox homoeostasis has been reported in various neoplastic disorders. Malondialdehyde/4-hydroxy-2,3-nonenal (MDA/HNE) and protein carbonyl groups represent in vivo indexes of lipid peroxidation and protein oxidation, respectively, suitable to investigate radical-mediated physio-pathological conditions. We evaluated MDA/HNE and protein carbonyl groups in sera of untreated ...

Background: Hubble-Bubble smoking is a common practice and spreading fast among young adults even in western countries. People think it less toxic than cigarette smoking.
 Aim: The aim of this study was to investigate the levels oxidative stress markers antioxidants blood (smokers compared controls as sign toxicity, since disease process caused by abnormal antioxidant capacity.
 Metho...

Carbonyl-carbonyl (C=O···C'=O') interactions are ubiquitous in both small and large molecular systems. This interaction involves delocalization of a lone pair (n) of a donor oxygen into the antibonding orbital (π*) of an acceptor carbonyl group. Analyses of high-resolution protein structures suggest that these carbonyl-carbonyl interactions prefer to occur in pairs, that is, one donor per accep...

Advanced glycation end products (AGE) include a variety of protein adducts whose accumulation has been implicated in tissue damage associated with diabetic nephropathy (DN). It was recently demonstrated that among AGE, glycoxidation products, whose formation is closely linked to oxidation, such as carboxymethyllysine (CML) and pentosidine, accumulate in expanded mesangial matrix and nodular les...

The effects of different concentrations gallic acid (0 mg/g, 1 2 4 and 6 mg/g) on the physicochemical properties Lateolabrax japonicas myofibrillar protein were studied with 400 W ultrasound. results showed that decreased particle size, total sulfhydryl group, carbonyl dimer tyrosine content Ca 2+ -ATPase activity (P<0.05) protein, however, increased zeta potential. Ultrasonic wave could coo...