Departme Tusa, N., J.W. Grosser, and F.G. Gmitter, Jr. 1990. Plant regeneration of ‘Valencia’ sweet orange, ‘Femminello’ lemon, and the interspecific somatic hybrid following protoplast fusion. J. Amer. Soc. Hort. Sci. 115:1043– 1046. Vardi, A., P. Arzee-Gonen, A. Frydman-Shani, and S. Bleichman. 1989. Protoplast fusion mediated transfer of organelles from Microcitrus and Citrus and regeneratio...

Cosynthesis in mixed culture and protoplast fusion of non-producing mutants of Streptomyces hygroscopicus which may produce biosynthetic intermediates of bialaphos (AMPBA) were studied. Non-producing mutants were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment, and six stable non-producing mutants were used for cosynthesis and protoplast fusion studies. The bio...

Cotton (Gossypium spp.) is an economically important crop that is grown throughout the world. It is the main material in textile and the main economic resource for more than 100 millions of cotton farmers in China. Due to its main role in our economy, cotton biotechnology has been significantly improved in the last decades in China, which led to a significantly progress both in cotton research ...

Plant protoplasts are significant for plant cell culture, somatic fusion, genetics, and breeding studies. In addition, in vitro regeneration has great importance developmental biology, manifesting potential applications agriculture biotechnology. this regard, we present a well-established protocol regarding protoplast isolation, culture fusion of Jasminum spp. particular, different tissues sama...

In vitro plant tissue culture and biotechnology used to assist support the development of breeding when classical methods propagation must be accelerated or it was necessary overcome barriers inaccessible by approaches. asparagus, improve multiple tasks, a high number in have been used, such as regeneration through organogenesis, embryogenesis, manipulation ploidy, protoplast isolation, genetic...

The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred μM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...

The ability to induce Arabidopsis protoplasts to dedifferentiate and divide provides a convenient system to analyze organelle dynamics in plant cells acquiring totipotency. Using peroxisome-targeted fluorescent proteins, we show that during protoplast culture, peroxisomes undergo massive proliferation and disperse uniformly around the cell before cell division. Peroxisome dispersion is influenc...

Brassica coenospecies has been bestowed with a large collection of wild and weedy relatives, which could act as donors of genes controlling agronomically important traits like disease resistance, yield attributes, modified fatty acid composition in the seed etc. Additionally, the cytoplasm of these species could be the sources for developing alloplasmic male sterility systems that are important...