نتایج جستجو برای: protoplast fusion
تعداد نتایج: 120504 فیلتر نتایج به سال:
Protoplasts from four different species of Streptomyces regenerated cells efficiently in hypertonic soft agar medium overlaid on partially dehydrated regeneration medium. The efficiencies of regeneration were strongly dependent upon the incubation temperatures for cell growth and for protoplast regeneration. Cell growth temperatures (before protoplast formation) required for efficient protoplas...
In vitro plant tissue culture and biotechnology used to assist support the development of breeding when classical methods propagation must be accelerated or it was necessary overcome barriers inaccessible by approaches. asparagus, improve multiple tasks, a high number in have been used, such as regeneration through organogenesis, embryogenesis, manipulation ploidy, protoplast isolation, genetic...
In this study, mutant forms of Bacillus thuringiensis spp. israelensis (H14) were produced. These mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. Protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. The protoplasts were induced to fuse to each other i...
Plant protoplasts are significant for plant cell culture, somatic fusion, genetics, and breeding studies. In addition, in vitro regeneration has great importance developmental biology, manifesting potential applications agriculture biotechnology. this regard, we present a well-established protocol regarding protoplast isolation, culture fusion of Jasminum spp. particular, different tissues sama...
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