Reactivity of the Co(III) complex of cyclen (CoCyc) in the hydrolytic cleavage of supercoiled pUC18 DNA leading to the formation of the corresponding open circular form was enhanced by >200 times upon attachment of CoCyc to cross-linked polystyrenes. Thus, half-lives as short as 40 min were achieved by the resin-based CoCyc in cleavage of the supercoiled DNA at 4 degrees C.

In EST projects it is extremely important to be able to identify the informative region of the read, by trimming the non informative ones. Several methods for sequence trimming are available. In this work we present a methodology to compare such methods, based on the sequencing of defined sequences (pUC18 cloning vector) and homology searches to find the informative region using BLAST. This met...

A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...

background: laron syndrome, is a disease that treated by igf-1. this protein is a single chain and has three disulfide bonds. people with laron syndrome have low rates of cancer and diabetes, although they appear to be at increased risk of casual death due to their stature. igf-1 is synthesized by many tissues and is secreted from liver as an endocrine hormone which is transmitted to other tiss...

We cloned the structural genes for the individual subunits of the branched-chain keto acid dehydrogenase multienzyme complex on a 7.8-kilobase EcoRI-SstI restriction fragment of Pseudomonas putida chromosomal DNA by cloning into the broad-host-range vector pKT230. A direct selection system for growth on valine-isoleucine agar was achieved by complementation of P. putida branched-chain keto acid...

Nanohydroxyapatite (n-HA)/zinc oxide (ZnO) complex was synthesized by a direct precipitation method, and the antibacterial capability and antibacterial mechanism of this complex were investigated in this article. Transmission electron microscope (TEM), Fourier transform infrared, X-ray photoelectronic spectroscopy (XPS), and X-ray diffraction (XRD) were used to analyze the materials. In additio...

The nucleotide sequence of a small (1273 bp) plasmid (pOX1000) of Staphylococcus aureus has been determined and compared with similar plasmids. The sequence includes a single open reading frame; two large palindromes and a 22 bp palindrome that is contiguously repeated three times upstream of the open reading frame. Composite plasmids of pE194ts and pOX1000 were constructed with pUC18 separatel...

Quantum dots (QDs) are inorganic fluorescent nanocrystals with excellent properties such as tunable emission spectra and photo-bleaching resistance compared with organic dyes, which make them appropriate for applications in molecular beacons. In this work, quantum dot-based molecular beacons (QD-based MBs) were fabricated to specifically detect β-lactamase genes located in pUC18 which were resp...