Quartz has a dissipation factor of approximately 10(-4), which is an order of magnitude less than that of the best glasses previously used to fabricate patch pipettes; it's dielectric constant of 3.8 is also lower than that of other glasses. On the basis of these electrical characteristics it is expected that patch pipettes pulled from quartz tubing will produce significantly less noise than pi...

background: successful embryo transfer (et) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. genetic ...

INTRODUCTIONThis protocol describes a method for concentrating gel bands or spots excised from 1D or 2D acrylamide gels. Several replicate protein bands (or spots) are often required to provide sufficient material for sequencing. In these situations, it is necessary to excise several stained protein bands or spots from the gels and pre-concentrate them prior to electroblotting for amino-termina...

A simplified method to manufacture durable, glass-insulated, tungsten microelectrodes with sufficient control of the final electrode impedance is described. This method requires only two instruments, an electrolytic etcher for wires and pipette puller, for manufacturing these electrodes. The manufacture of these electrodes involves 3 steps: (1) etching tungsten wire to sharpen the tip, (2) insu...

In this paper, an investigation on surface properties of glass micropipettes and their effect on biological applications is reported. Pipettes were pulled under different pulling conditions and the effect of each pulling parameter was analyzed. SEM stereoscopic technique was used to reveal the surface roughness properties of pipette tip and pipette inner wall in 3D. More than 20 pipettes were r...

The olfactory mucosa of the frog was isolated, folded (the outer, ciliated side faced outward), and separately superfused with Ringers solution on each side. A small number of sensory cilia (one to three) were pulled into the orifice of a patch pipette and current was recorded from them. Fast bipolar current transients, indicating the generation of action potentials by the receptor cells, were ...

Diabetes mellitus is a metabolic disease that has posed public health problem for long time and whose medical treatment lifelong expensive. Thus, populations have the alternative of using medicinal plants. The aim this work was to evaluate antidiabetic activity aqueous extract trunk bark Sclerocarya birrea (EAqScB) its cyclohexane fraction (F4) in order justify traditional use diabetes mellitus...

BACKGROUND Successful Embryo Transfer (ET) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. Small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. Genetic a...

Treatment of human African trypanosomiasis (HAT) due to Trypanosoma brucei gambiense requires distinction between haemo-lymphatic and meningo-encephalitic stages. The presence of trypanosomes in the cerebrospinal fluid (CSF) is not only an evidence for diagnosis, but also indicates central nervous system invasion (WHO, 1998). When their number in CSF is high, trypanosomes are detectable during ...