The transient receptor potential (TRP) family of proteins are generally nonselective cation channels that participate in many sensory and physiological processes. To date, no structural information at the atomic level is available for any full-length TRP channels due to difficulties encountered in overexpression, functional puri-fi cation, and crystallization of eukaryotic transmembrane protein...

Protein biologics now represent a signifi cant share of pharmaceutical research and development efforts. Their specifi city has been their key to success, as their cell-specifi c modality and relative lack of compound-specifi c toxicity have provided safer and more effective means to treat several chronic diseases. The most common class of proteins used as pharmaceuticals is monoclonal antibodi...

However, the co-existence of metallic and semiconducting tubes in as-synthesized SWNT samples has been a bottleneck for fundamental research and fabrication of electronic and optoelectronic devices. [ 6 ] It is desirable to develop effective purifi cation methods by which SWNT samples enriched in semiconducting species may be prepared. Various methods to sort semiconducting from metallic nanotu...

Aerogels are porous, low density (0.8–0.001 g cm −3 ) solid materials with a high specifi c surface area; they have been recognized as promising materials for a plethora of applications, such as transparent thermal or acoustic insulation, membrane separation, lightweight construction, supported catalysis, superabsorbency, and water purifi cation. [ 1–10 ] Advanced mateAmbient-Dried Cellulose Na...

06 Transparent conductive oxides (TCOs) are a technologically important class of materials. [ 1 ] Herein we describe a facile, universal, and green ‘one-pot’ approach to produce stable dispersions comprising TCO nanoparticles (NPs) such as SnO 2 , In 2 O 3 , ATO ( ≡ SnO 2 :Sb), ITO ( ≡ In 2 O 3 :Sn), and ZTO ( ≡ SnO 2 :Zn). The synthesis begins by etching the bare metal powder precursors (Sn, I...

Removal Of Host Cell Proteins By Zeta PlusTM Filters During Monoclonal Antibody Purifi cation Introduction Protein A chromatography is a widely used and highly successful method of purifying recombinant monoclonal antibodies. However, since Protein A has high affi nity for antibodies, harsh conditions such as low pH are often required to elute monoclonal antibodies from Protein A affi nity colu...

Preparation of radioantibodies from carcinoembryonic anti gen (CEA) antisera raised in goats by conventional procedures necessitates extensive absorption procedures to remove anti body activity against CEA-related substances. Further purifi cation by affinity chromatography increases the antibody con tent of the radiolabeled preparation and enhances antibody uptake by a xenogeneic tumor. In pat...

Background: Immunoglobulin E–mediated allergies have doubled in prevalence during recent decades in developed countries. This increase has been attributed, in part, to high hygiene standards, which have reduced exposure to microbes. The capacity of microbes to induce type 1 helper T cell (TH1) responses may imply suppression of TH2 responses. However, little research has been performed with fun...

Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...