نتایج جستجو برای: pvax1

تعداد نتایج: 64  

Journal: :Experimental animals 2010
Fuying Liu Jingli Shang Shuxia Song Long Zheng Kaixia Zhang Hongyu You Huanling Zhang Junxia Wang

The objective of this study was to obtain better antigen specific cytotoxic T cell responses in vivo. We examined the augmented induction of antigen-specific cytotoxic T cell responses to co-administration of oligonucleotides (CpG-ODN), dimethyl dioctadecyl ammonium bromide (DDA), and Lipofectamine™ 2000 with a DNA vaccine (pVAX1-CpG-Loop) and boosting with pVAX1-CpG-Loop in BALB/c mice. The re...

2015
Rahman Abdizadeh Sharif Maraghi Ata A. Ghadiri Mehdi Tavalla Saeedeh Shojaee

BACKGROUND Toxoplasmosis is an opportunistic protozoan infection with a high prevalence in a broad range of hosts infecting up to one-third of the world human population. Toxoplasmosis leads to serious medical problems in immunocompromised individuals and fetuses and also induces abortion and mortality in domestic animals. Therefore, there is a huge demand for the development of an effective va...

Journal: :The Onderstepoort journal of veterinary research 2013
Tingting Cui Jun Xiong Yongzhi Wang Xintian Wen Xiaobo Huang Yong Huang Xiaoping Ma Zhongkai Hu Qin Zhao Sanjie Cao

The attenuated Salmonella typhimurium χ4550 strain was used to harbour a reconstructed bicistronic DNA vaccine against porcine rotavirus, which carried the rotavirus nonstructural protein 4 (NSP4) and VP7 genes simultaneously. Using a balanced lethal system, the kanamycin resistance gene of expressing eukaryotic plasmids pVAX1 and pVAXD were replaced by the aspartate β-semialdehyde dehydrogenas...

2017
Bin Zheng Jianzu Ding Xiaoheng Chen Haijie Yu Di Lou Qunbo Tong Qingming Kong Shaohong Lu

Toxoplasma gondii (T.gondii) is distributed worldwide and infects most species of warm-blooded animals, including humans. Toxoplasmosis has serious consequences, especially in people with an impaired or immature immune system. Thus, an effective vaccine is urgently required. Secretory microneme proteins are essential for the adhesion and invasion of T. gondii. The gene encoding the microneme pr...

2017
Xiaoyan Zheng Hui Chen Ran Wang Dongying Fan Kaihao Feng Na Gao Jing An

Dengue virus (DV) is the causal pathogen of dengue fever, which is one of the most rapidly spread mosquito-borne disease worldwide and has become a severe public health problem. Currently, there is no specific treatment for dengue; thus, a vaccine would be an effective countermeasure to reduce the morbidity and mortality. Although, the chimeric Yellow fever dengue tetravalent vaccine has been a...

2013
Man Li Yuhong Jiang Chaoqun Xu Zhirong Zhang Xun Sun

BACKGROUND The heterologous deoxyribonucleic acid (DNA) prime-adenovirus (AdV) boost vaccination approach has been widely applied as a promising strategy against human immunodeficiency virus (HIV)-1. However, the problem of inefficient delivery and lack of specificity of DNA vaccine remains a major issue. In this paper, to improve the transfection of DNA vaccine and realize dendritic cell targe...

2017
Lei Wang Ying Liu Jingrui Huo Yuanyuan Liu Yi Tian Xiaohui Yang Jingjing Zhang Yingfu Liu

The effectiveness of DNA vaccination alone is limited, because it often generates only a weak cellular immune response; therefore, the complementary use of adjuvants may be required to improve vaccine potency and enhance its immune protective effects. The present study aimed to determine the effect of recombinant DNA vaccine-based human hcg and survivin on the development of breast carcinoma in...

2017
Yuan-Feng Gong Quan-Bo Zhou Ya-Di Liao Cong Mai Tie-Jun Chen Yun-Qiang Tang Ru-Fu Chen

Considering mucin 1-variable number tandem repeat (MUC1-VNTRn) as a novel target for pancreatic cancer immunotherapy, the present study aimed to screen and identify the pVAX1-MUC1-VNTRn DNA vaccine with the strongest immunogenicity. Following construction of a pVAX1-MUC1-VNTRn plasmid, immature dendritic cells (DCs) were subjected to transfection, and mature DCs were then co-cultured with autol...

Journal: :iranian journal of parasitology 0
f chen the faculty of life sciences, hubei university, 368 youyi road, wuchang, wuhan 430062, china q liu hainan provincial key laboratory of tropical medicine, pharmacy school, hainan medical college, haikou 571199, china y xue lab of medical engineering, college of medical technology and engineering, henan university of science and techology, luoyang 471003, china yh huang hainan provincial key laboratory of tropical medicine, pharmacy school, hainan medical college, haikou 571199, china fy huang hainan provincial key laboratory of tropical medicine, pharmacy school, hainan medical college, haikou 571199, china y lin hainan provincial key laboratory of tropical medicine, pharmacy school, hainan medical college, haikou 571199, china

background : malaria remains a serious public health problem with significant morbidity and mortal-ity. this study was conducted to identify whether ficolin-a could play an active role of against mala-ria infection. methods : the function of ficolin-a was analyzed in mouse model. the open reading frame of fico-lin-a was cloned from the liver of new born c57bl/6 mice by rt-pcr and then inserted ...

2017
Liqing Tu Pei Zhou Lutao Li Xiuzhen Li Renjun Hu Kun Jia Lingshuang Sun Ziguo Yuan Shoujun Li

Canine influenza virus (CIV) has the potential risk to spread in different areas and dog types. Thus, there is a growing need to develop an effective vaccine to control CIV disease. Here, we developed three vaccine candidates: 1) a recombinant pVAX1 vector expressing H3N2 CIV hemagglutinin (pVAX1-HA); 2) a live attenuated canine adenovirus type 2 expressing H3N2 CIV hemagglutinin (rCAV2-HA); an...

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