BACKGROUND Hepatic ischemia-reperfusion (I/R) injury is a pivotal clinical problem occurring in many clinical conditions such as transplantation, trauma, and hepatic failure after hemorrhagic shock. Apoptosis and autophagy have been shown to contribute to cell death in hepatic I/R injury. Ethyl pyruvate, a stable and simple lipophilic ester, has been shown to have anti-inflammatory properties. ...

deprotection of phosphate, phosphate, and sulfite esters by silica chloride is described. silica chloride is a mild, selective, and effective reagent for cleavage of benzyl and t-butyl esters of the above compounds.

BACKGROUND Human African Trypanosomiasis (HAT) also called sleeping sickness is an infectious disease in humans caused by an extracellular protozoan parasite. The disease, if left untreated, results in 100% mortality. Currently available drugs are full of severe drawbacks and fail to escape the fast development of trypanosoma resistance. Due to similarities in cell metabolism between cancerous ...

that, under anaerobic conditions, appreciable quantities of pyruvate were metabolized by Flex ner-,Jobling carcinoma homogenates to reduced compounds other than lactic acid. For these stud ies a medium of glycolytic substrates and cofactors was employed in the presence of a “fluorideblock.― This medium permitted the quantitative estima tion of pyruvate metabolism to products other than lact...

that, under anaerobic conditions, appreciable quantities of pyruvate were metabolized by Flex ner-,Jobling carcinoma homogenates to reduced compounds other than lactic acid. For these stud ies a medium of glycolytic substrates and cofactors was employed in the presence of a “fluorideblock.― This medium permitted the quantitative estima tion of pyruvate metabolism to products other than lact...

Pyruvate decreased the rate of free fatty acid release from rat epididymal adipose tissue incubated in vitro by increasing free fatty acid esterification. The magnitude of the pyruvate effect, tested in tissues from fasted, adrenalectomized, or diabetic rats, closely correlated both with the rate of glyceride-glycerol labeling from radioactive pyruvate and with the activity of P-enolpyruvate ca...

that, under anaerobic conditions, appreciable quantities of pyruvate were metabolized by Flex ner-,Jobling carcinoma homogenates to reduced compounds other than lactic acid. For these stud ies a medium of glycolytic substrates and cofactors was employed in the presence of a “fluorideblock.― This medium permitted the quantitative estima tion of pyruvate metabolism to products other than lact...

Pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and ADP to yield ATP and Pyruvate. Geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. Given that limited proteolysis experiments can be successfully used to probe conformational features of p...

2,3-dihydroxy-2-methylbutyric acid, also known as 2,3-dimethylglyceric constitutes the acyl and/or alcoholic moiety of many bioactive natural esters. Herein, we describe a chemoenzymatic methodology which gives access to all four possible stereoisomers acid ethyl ester. The racemic ?-acetolactate, produced by N-heterocycle carbene (NHC)-catalyzed coupling pyruvate and methylacetoin was employed...

Heat induced complications cause an increase in a large number of proteins which play a role in diverse pathways during heat shock. A detailed characterization of these proteins is essential for understanding the molecular mechanisms involved in heat stroke. In this report, the proteins present in rat liver were compared at 37 degrees C (control) and at core temperature (Tc) 42 degrees C (heat ...