The Agilent 6550 iFunnel Q-TOF LC/MS System (6550 Q-TOF) has been coupled with fl ow injection analysis (FIA) to provide profi ling of small molecules in 96 biological extracts. This experiment demonstrates the superior performance of this platform, compared to the results produced with a previous generation Q-TOF instrument, resulting in the detection of an order of magnitude more ions. The in...

Sample pretreatment was optimized to obtain high sequence coverage for human serum albumin (HSA, 66.5 kDa) when using nano-electrospray ionization quadrupole time-of-flight mass spectrometry (nESI-Q-TOF-MS). Use of the final method with trypsin, Lys-C, and Glu-C digests gave a combined coverage of 98.8%. The addition of peptide fractionation resulted in 99.7% coverage. These results were compar...

Inductively coupled plasma mass spectrometry (ICP-MS) is a suitable tool for multi-element analysis at low concentration levels. Rare earth element (REE) determinations in standard reference materials and small volumes of molten ice core samples from Antarctica have been performed with an ICP-time of flight-MS (ICP-TOF-MS) system. Recovery rates for REE in e.g. SPS-SW1 amounted to approximately...

The metabolic profiles of Panax quinquefolius and its associated therapeutic values are critically affected by the repetitious steaming times. The times-dependent steaming effect of P. quinquefolius is not well-characterized and there is also no official guideline on its times of steaming. In this paper, a UPLC-Q-TOF-MS/MS method was developed for the qualitative profiling of multi-parametric m...

The Agilent 6540 Q-TOF LC/MS and Agilent Mass Profiler Professional Software (MPP) were used to sensitively characterize the complex chemical composition of municipal wastewater. The processing of Q-TOF high-resolution MS data using the MPP multivariate statistical analysis package revealed changes in the occurrence patterns of organic chemicals during water treatment. Using this technique, we ...

BACKGROUND Coptis chinensis Franch is a traditional Chinese medical herb. OBJECTIVE In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. MATERIALS AND METHODS Chromatographic separation was achieved on an Agilent Zorbax RRHD...