A soluble enzyme system which catalyzes the aerobic oxidation of reduced pyridine nucleotides in wheat germ has previously been described (1). Peroxidase was shown to be a component of this system. Later, Humphreys (2) demonstrated that Mn+ and an unidentified cofactor were required for aerobic oxidation of TPNH’ in crude preparations of malic enzyme from wheat germ. Although no attempt was mad...

The occurrence of immunoglobulins with and without antibody specificity and with and without idiotypic specificity was studied, by use of enzyme-labeled antigen and antibodies, in lymph node cells of rabbits immunized with horse-radish peroxidase and hen ovalbumin. Some cells, containing immunoglobulins without detectable antibody function, were shown to contain idiotypes similar to those found...

Syringaldehyde readily reacted in the horse-radish peroxidase (HRPOD) system. The ethyl acetate extract of the reaction mixture showed a marked antimicrobial activity against bacteria and fungi. After repeated column chromatography three potential antimicrobial compounds were obtained from the extract. The structural elucidation of active compounds was achieved by a combination of spectroscopic...

Retinotectal fibres from different parts of the retina have been filled with Horse Radish Peroxidase (HRP). During their course down the nerve their ordering decays, but it is re-established at the chiasma, except for nasal fibres which remain relatively disordered. The consequences of this for development are discussed.

The effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxidase (POD; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1), ascorbic peroxidase (APOX; EC 1...

Oxidation of LDL by peroxidases has been suggested to be a model for in vivo oxidation. The mechanism might involve the generation of an intermediate radical such as a phenoxy radical. We show that, in contrast to the oxidation of LDL by copper, oxidation by peroxidase system (H2O2/horse radish peroxidase) showed less resistance. This suggested that either the antioxidants were consumed more ra...

IAULLFR (1) first demonstrated that fl-glucose oxidase promoted the oxidation of glucose by molecular oxygen to gluconic acid. Franke and Lorenz (2) discovered that hydrogen peroxide is simultaneously produced in this reaction. Keston (3), Comes (4), and Free et at. (5) made the first practical application of the use of glucose oxidase in the estimation of glucose in urine by impregnating paper...

Arsenic (As) pollution, a potential threat for human health, in vegetables is one of the primary sources As intake by body. In Pot Experiment, content, physiological index and antioxidant enzyme activity radish were determined. The results demonstrated that order concentration tissues was roots > stems leaves. Organic selenium (Se) can inhibit absorption arsenic more effectively than inorgan...