The optimum conditions for in vitro iodination of RNAs have been established which yield specific radioactivities ranging from 10 x 10(4) to 10 x 10(6) cpm/mug. A nomogram has been constructed by correlating specific radioactivities of RNA with concentration of KI, RNA, and (125)I. This nomogram can be used to determine the conditions for the desired specific radioactivities for any unknown RNA...

After intravenous administration of [3H-heme,14C-globin]hemoglobin.haptoglobin or [59Fe-heme]hemoglobin.haptoglobin to rats, the radioactive materials extracted from the liver homogenate or its subcellular fractions were subjected to a gel filtration column. In addition to the 82-kDa component, we found three metabolites of heme moiety of the complex in the subcellular fractions. Intact hemoglo...

Rat hepatocytes were incubated with 14C-labelled hexoses, and the specific radioactivities of glucose 6-phosphate, glucose 1-phosphate and fructose 6-phosphate were determined. (1) When suspensions of freshly isolated hepatocytes were incubated with [14C]glucose, the specific radioactivities of glucose 1-phosphate and fructose 6-phosphate were severalfold higher than that of glucose 6-phosphate...

The radioactivity concentrations of 40 K, 238 U, 232 Th and 137 Cs in eighteen species of foodstuffs in the oil and gas producing areas in Delta State, Nigeria have been measured using a high purity germanium, (HPGe) detector system. The mean specific activities of 40 K, 238 U, 232 Th and 137 Cs vary between 36.48 – 68.02; 2.33 – 5.18; 1.26 – 2.98 and 0.27 – 2.47 Bq kg -1 respectively in vegeta...