A new cell line “CK cell line” capable of continuous propagation was established from a calf kidney tissue. The bovine adenovirus type 3 could propagate well in this cell line. ∗PMID: 4280232 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med. Okayama 28, 213-217 (1974) A NEW CELL LINE ESTABLISHED FROM CALF KIDNEY Makoto MOTO! Department of Pathology, Okayama U...

Herein, we presented the first report on bluetongue (BT) disease in 10 pregnant camels in a herd fromKerman province, Iran. All sera samples were tested serologically (AGID, C-ELISA). We also used the Razi-BK cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ECE) to culture and isolate the BT virus. Efforts to culture and isolation of...

herein, we presented the first report on bluetongue (bt) disease in 10 pregnant camels in a herd fromkerman province, iran. all sera samples were tested serologically (agid, c-elisa). we also used the razi-bk cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ece) to culture and isolate the bt virus. efforts to culture and isolation of...

this report describes the preparation of a safe and immunogenic single vaccine against capripox disease in sheep and goats using two different types of cells (primary lamb kidney and vero, a bovine turbinate cell line). this used the 0240 pox strain isolated from sheep during an outbreak. the prepared vaccine was administered subcutaneously and induced complete protection against an experimenta...

nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. the number of studies on the role of schwann cells (sc) in treating nervous disorders is higher than other cell types. different protocols have been suggested for isolation and expansion of sc which most of them have used multiple growth factors, mitogens and fetal b...

This report describes the preparation of a safe and immunogenic single vaccine against capripox disease in sheep and goats using two different types of cells (primary lamb kidney and vero, a bovine turbinate cell line). This used the 0240 pox strain isolated from sheep during an outbreak. The prepared vaccine was administered subcutaneously and induced complete protection against an experimenta...

enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (sscs) transplantation. knock-out serum replacement (ksr) has been reported to enhance the proliferation of murine sscs and human embryonic stem cells. the aim of the present study was to investigate the effect of ksr versus fetal bovine serum (fbs) ...

During an investigation of bovine brucellosis in Iran, conducted by the Razi Institute over a twelve-month period, samples of serum and milk were collected simultaneously from 6,472 cows in eight infected herds for serological and bacteriological testing. A total of 1,056 cows were serologically positive and 1,632 of 6,472 milk samples were positive to the milk ring test (MRT). Culture of the p...

Microneutralization tests for detection of antibody in bovine serum to three bovine viruses are described. The Madin-Darby bovine kidney cell line was used with parainfluenza 3 virus (PI 3), whereas serially cultivated bovine embryonic kidney cells were used for infectious bovine rhinotracheitis virus and bovine virus diarrhea virus. Comparison of micro-hemagglutination-inhibition (HI) with mic...

Primary bovine embryonic kidney cell was successfully replaced by the green monkey cell line (Vero) for the isolation and seroneutralization of rinderpest virus.