background: existence of bacterial host cell dna contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. hence, the quantity of contamination must be controlled under the regulatory standards. although different methods such as hybridization assays have been employed to determine dna impurities, these methods are labor, intensive and rather exp...

background: mycobacterium tuberculosis is the causative agent of tuberculosis (tb). bacille calmette-guerin (bcg) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult tb vaccine. the aim of this study was to develop a new tuberculosis dna vaccine candidate encoding a recombinant hspx-ppe44-esxv fusion antigen of m. tuberculosis. methods: a fu...

The Nobel laureate Paul Berg initiated genetic manipulation and pioneered engineering. He was also one of the initiators setting up guidelines for carrying out recombinant DNA experiments. an innovative scientist cared impact science on society.

  Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...

background: serological assay based on dense granular (gra) proteins of toxoplasma gondii (t. gondii) is actually the most popular laboratory diagnostic tool to detection of toxoplasmosis. we aimed to construct a recombinant gra7-ptz57rt plasmid vectors that it is suitable for sub-cloning and gra7 protein production. m aterials and methods: souris mice were used for maintaining of t. gondii tac...