background: detection of hepatitis c virus specific antibodies is the initial step in chronic hcv diagnosis.  hcv ns4b is among the most immunogenic hcv antigens and has been widely used in commercial enzyme immunoassays (eia). additionally, ns4b, a key protein in the virus replication, can be an alternative target for antiviral therapy.objectives: development of a new method for high-level exp...

background: regarding that accurate diagnosis of human hydatidosis still needs more investigations, the present study was conducted to clone, express, and evaluate the gene encoding agb subunits (egagb16 kda) from echinococcus granulosus (iranian g1 strain) and its evaluation by elisa test. methods: dna was extracted from protoscoleces and was utilized by pcr for strain identification. total rn...

the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...

Bovine Viral Diarrhea virus (BVDV) is an important viral pathogen of cattle causing several clinical syndromes. There are usually no pathognomonic clinical signs of BVDV infection. Diagnostic investigations therefore rely on serological detection and virus isolation. Nonstructural protein 3 (NS3) as immunogenic protein of BVDV is genetically and antigenically conserved among different isolates....

The cyclic voltammetric behaviour of copper (II) tetraethylethylenediamine systems with [Cu(II)]:[teen] =1:10, 1:100 molar ratios, 1x10M Cu(ClO4)2.6H2O in aqueous 0.2M NaClO4 supporting electrolyte have been studied at pH7.20, 9.10 and 10.85. The electrode process show quasi reversible one electron charge transfer preceded and followed by chemical reaction at glassy carbon electrode (GCE). For ...

background: dense granules are immunodominant proteins for the standardi-zation of immunodiagnostic procedures to detect neosporosis. in the presented study different fragment of a dense-granule protein was evaluated for serodiagno-sis of neospora caninum in cattle and water buffalo. methods: ncgra7, from n. caninum tachyzoites was amplified. pcr product and pmal-c2x plasmid were digested with ...

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...

Objective(s): Production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. The goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. Materials and Methods: Multiple amino acid sequences of different i...