We demonstrate that the second-Stokes output from a diamond Raman laser, pumped by a femtosecond Ti:Sapphire laser, can be used to efficiently excite red-emitting dyes by two-photon excitation at 1,080 nm and beyond. We image HeLa cells expressing red fluorescent protein, as well as dyes such as Texas Red and Mitotracker Red. We demonstrate the potential for simultaneous two-color, two-photon i...

In the recent years, laser ablation in liquid has become an increasingly important technique for the fabrication of NPs. this paper reports our recent studies on the generation of ZnO NPs by ablation of metal targets in aqueous environments using Q-switch Nd-YAG laser (λ=532nm) immersed in NaOH (0.1M). The Surface topography studied by atomic force microscopy revealed wider size distributio...

Laser is one of the sources that induce a biological effect in higher plants. The positive effect of laser on some crops has been proved. This research was carried out to study the effect of laser radiation on the accumulation of proline in spring wheat (Triticum aestivum cv. Kavir) under different NaCl treatment levels. Semi-coherent red beam, infrared beam and one-second harmonics of Neodymiu...

Diode and DPSS lasers emitting a variety of wavelengths are now commonly incorporated into flow cytometers, greatly increasing our capacity to excite a wide variety of fluorochromes. Until recently, however, virtually no practical technology existed for generating yellow or orange laser light for flow cytometry that was compatible with smaller instrumentation. In this study, we evaluate several...

Light is an effective factor in cell suspension culture and must be controlled for optimizing cell growth. Growth of anthocyanin producing suspension cells of a red-fleshed genotype of apple (RFA) was assessed in response to blue diode laser (BDL) and red He Ne (RHNL) laser. The suspension cells in L-shaped test tubes were exposed to short-term laser radiation for 20 min in a rotary shaker. The...

vascular lesions rise from abnormalities in blood vessels or endothelial proliferation . capillary hemangiomas are formed by small capillaries surrounded by a layer of endothelial cells in a connective tissue stroma . various treatments are used for these conditions like excisional surgery , sclerotherapy, and recently laser irradiation. in this case study, we report successful treatment of int...

introduction: low-level laser therapy (lllt) is shown effective in healing diabetic foot ulcer when combined with conventional therapy. since; diabetes mellitus is a leading cause of impaired wound healing. the aim of this study is to determine the effect of different diabetic control’s methods on the healing response of diabetic foot ulcer in those patients treated with low level laser therapy...

Low-level lasers are used at low power densities and doses according to clinical protocols supplied with laser devices or based on professional practice. Although use of these lasers is increasing in many countries, the molecular mechanisms involved in effects of low-level lasers, mainly on DNA, are controversial. In this study, we evaluated the effects of low-level red lasers on survival, fila...

Single-color illumination of a copper surface by a red or an ultraviolet femtosecond laser pulse yields a threephoton (red) or a two-photon (UV) photoemission process. A multicolor, multiphoton process is generated when the red and the UV pulses overlap both in space and in time on the photocathode. It is shown that this emission process results from the absorption by an electron of one red and...