Providing transparent replication of servers has been a major goal in the fault tolerance community. Transparent replication is particularly challenging for highly nondeterministic applications, such as the ones that use multithreading. For such applications, keeping replicas in a consistent state becomes non-trivial. One way to deal with the non-determinism is to use a leader/follower approach...

Polymerase chain reaction (PCR) is largely used in molecular biology for increasing the copy number of a specific DNA fragment. The succession of 20 replication cycles makes it possible to multiply the quantity of the fragment of interest by a factor of 1 million. The PCR technique has revolutionized genomics research. Several quantification methodologies are available to determine the DNA repl...

We have analyzed the effect of flanking sequences on Tus-induced replication arrest. pBR322 plasmid derivatives which carry the Escherichia coli replication terminator TerB at different locations were used. Efficiency of the replication arrest was estimated from the plasmid copy number and transformation frequency of tus+ cells. We found that flanking sequences do affect replication arrest effi...

Abstract— Data grids are an important branch of gird computing which provide mechanisms for the management of large volumes of distributed data. Energy efficiency has recently emerged as a hot topic in large distributed systems. The development of computing systems is traditionally focused on performance improvements driven by the demand of client's applications in scientific and business domai...

The hepatitis C virus (HCV) genotype 2a isolate JFH1 represents the only cloned HCV wild-type sequence capable of efficient replication in cell culture as well as in vivo. Previous reports have pointed to NS5B, the viral RNA-dependent RNA polymerase (RdRp), as a major determinant for efficient replication of this isolate. To understand the contribution of the JFH1 NS5B gene at the molecular lev...

In this paper, we describe how, in a model embryonic system, cyclin-dependent kinase (Cdk) activity controls the efficiency of DNA replication by determining the frequency of origin activation. Using independent approaches of protein depletion and selective chemical inhibition of a single Cdk, we find that both Cdk1 and Cdk2 are necessary for efficient DNA replication in Xenopus egg extracts. E...

A wireless Sensor Network transfers the data one node to another node, energy efficient manner. The captured node, and place these replicas back into strategic positions in the network for further malicious activities. This is a so called node replication attack. The Range Based Detection Method (RBDM) to replication attacks are proposed to resist node replication attacks in mobile sensor netwo...