Due to their ability to integrate into the host cell's genome, retroviruses represent an optimal basis for the creation of gene therapy vectors. The integration reaction is carried out by a viral enzyme integrase: thus, a detailed research of this enzyme is required. In this work, the catalytic properties of human foamy virus integrase were studied. This virus belongs to the Retroviridae family...

The ectodomain of the Ebola virus Gp2 glycoprotein was solubilized with a trimeric, isoleucine zipper derived from GCN4 (pIIGCN4) in place of the hydrophobic fusion peptide at the N terminus. This chimeric molecule forms a trimeric, highly alpha-helical, and very thermostable molecule, as determined by chemical crosslinking and circular dichroism. Electron microscopy indicates that Gp2 folds in...

Interferons (IFNs) exert their anti-viral effects by inducing the expression of hundreds of IFN-stimulated genes (ISGs). The activity of known ISGs is insufficient to account for the antiretroviral effects of IFN, suggesting that ISGs with antiretroviral activity are yet to be described. We constructed an arrayed library of ISGs from rhesus macaques and tested the ability of hundreds of individ...

Budding is the final step of the late phase of retroviral life cycle. It begins with the interaction of Gag precursor with plasma membrane (PM) through its N-terminal domain, the matrix protein (MA). However, single genera of Retroviridae family differ in the way how they interact with PM. While in case of Lentiviruses (e.g., human immunodeficiency virus) the structural polyprotein precursor Ga...

1.1.1 Taxonomy The human immunodeficiency virus type 1 (HIV-1) was first isolated in 1983 (BarréSinoussi et al., 1983; Gallo et al., 1983), and firmly associated with the acquired immunodeficiency syndrome (AIDS) in 1984 (Gallo et al., 1984; Montagnier et al., 1984). A second related virus, called HIV-2, was subsequently discovered in West Africa (Clavel et al., 1986). HIV-1 and HIV-2 belong to...

Human immunodeficiency virus (HIV) is a member of the Retroviridae (retrovirus) family. It associated with immunodeficiency, neurological diseases and neoplasms. With use antiretroviral therapy (ART), prognosis people who are infected HIV has improved, incidence HIV-related central nervous system (CNS) markedly decreased. In spite that mortality from CNS remains significant. Magnetic resonance ...

Foamy viruses (FV) belong to the genus Spumavirus, which forms a distinct lineage in the Retroviridae family. Although the infection in natural hosts and zoonotic transmission to humans is asymptomatic, FVs can replicate well in human cells making it an attractive gene therapy vector candidate. Here we present cryo-electron microscopy and (cryo-)electron tomography ultrastructural data on purif...

Background: AIDS (Acquired Immunodeficiency Syndrome) is a collection of symptoms or diseases caused by decrease in the immune system from body due to infection HIV (Human Virus) which belongs retroviridae family. Teenagers themselves are vulnerable HIV/AIDS. The right and precise knowledge important prevention efforts adolescents. Method: This research descriptive with cross-sectional design. ...