ObjectiveTo screen pregnant women at risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during delivery using reverse-transcription polymerase chain reaction (RT-PCR) test and serum immunoglobulin (Ig) testing.MethodBetween March 31 st August 2020, consecutive admitted for labor in a single hospital were screened SARS-CoV-2 with nasopharyngeal RT-PCR swab tests detec...

Abstract Background Respiratory tract infections are the most common that lead to morbidity and mortality worldwide. Early recognition precise diagnosis of microbial etiology is important treat LRTIs promptly, specifically effectively. Objectives To establish a method based on multiplex reverse transcription (MRT)-PCR MassARRAY technology for simultaneous detection 27 respiratory pathogens expl...

Purpose: Prove that Computed Tomography (CT) is a more effective diagnostic tool for diagnosing Coronavirus Disease 2019 (COVID-19) in comparison with Reverse Transcription Polymerase Chain Reaction (RT-PCR) due to its higher sensitivity.
 Methods and materials: A total of 200 cases had records chest CT scans RT-PCR tests were collected from Palestinian governmental hospitals September Nov...

Background: This study aimed to evaluate the diagnostic value of outer dense fiber 4 (ODF4), melanoma-associated antigen A3 (MAGEA3), and MAGEAB4 mRNAs in transitional cell carcinoma (TCC), using a small amount of cell reverse transcriptase-polymerase chain reaction (RT-PCR) on urinary exfoliated cells. Methods: We recruited a total of 105 suspected TCC patients and 54 sex- and age-matched non-...

Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and ammonia monooxygenase gene expression in situ. The inhibitory effect of RT on the PCR was removed with increasing template concentrations beyond 10(5) to 10(6) copies. Including T4 gene 32 protein during the reverse trans...

Introduction: The novel coronavirus disease 2019 (COVID-19) has huge impact on public health. RT-PCR of respiratory samples is generally accepted confirmatory test which can miss several cases due to various factors. Case description: A 32-years-old male without any co-morbidity presented with complaints cough and fever was negative for Reverse Transcription Polymerase Chain Reaction (RT-PCR) t...

In this study, we report a familial cluster of cases which included five patients and two close contacts who were confirmed to have coronavirus disease 2019 (COVID-19). These participants had received real-time reverse transcription-polymerase chain reaction (RT-PCR) chest X-rays (CXRs) before diagnosis. The follow-up CXRs three in the family showed significant progression, with COVID-19 pneumo...

The use of reverse transcriptase coupled with the polymerase chain reaction (RT-PCR) has been proven to be a valuable tool in the synthesis and isolation of cDNA clones as well as the quantification of rare messenger RNA species. While a number of different approaches can be used to permit gene-specific cDNA synthesis and amplification, a recurring problem is one of high background signal (i.e....