Effects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solutes with known descriptors by computer using the program SPSS/PC. The experimental results showed t...

a high performance liquid chromatography method was studied for determination of 18 ?-glycyrrhetinic acid in glycyrrhiza glabra l. (licorice) extract. the operating condition were c-18 reversed phase column (vp-ods, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and uv detection at 230 nm. the r...

Poly(L-lactic acid) is a linear aliphatic thermoplastic polyester that can be produced from renewable resources. A poly(L-lactic acid)-modified silica stationary phase was newly prepared by amide bond reaction between amino groups on aminopropyl silica and carboxylic acid groups at the end of the poly(L-lactic acid) chain. The poly(L-lactic acid)-silica column was characterized in reversed-phas...

the use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (sds) and polyoxyethylene (23) dodecanol (brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. a group of chlorophenols was used as the test mixture. adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in sds/brij-35 mixe...

High-temperature liquid chromatography (HTLC) is recognized today as a valuable technique in reversed-phase high-performance liquid chromatography (RP-HPLC). Column temperature can play a role in reducing analysis time, modifying retention, controlling selectivity, changing efficiency or improving detection sensitivity. The different effects of high temperatures on reversed-phase separations, t...

Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons fo...

Polarity Reversed-phase chromatography and hydrophobic-interaction chromatography Centrifugation: Analytical Centrifugation. Chromatography: Protein Separation. III/Enzymes: Liquid Chromatography. Proteins: Capillary Electrophoresis; Centrifugation; Crystallization; Electrophoresis; Glycoproteins: Liquid Chromatography; High-Speed Countercurrent Chromatography; Ion Exchange; Metalloproteins: Ch...

Abstract The application of temperature gradient interaction chromatography (TGIC) as an advanced technique for the characterisation polymers is discussed, in comparison to other liquid techniques and particular ubiquitous size exclusion chromatography. Specifically, use reversed-phase TGIC interrogation complex branched normal-phase high-molar mass end-functionalised highlighted.