1. The reversible dissociation of lactic and malic dehydrogenases has been studied in detail and compared with the dissociation properties of triosephosphate dehydrogenase, a-glycerophosphate dehydrogenase, and liver alcohol dehydrogenase. 2. Evidence is presented for the existence of an intermediate form of lactic dehydrogenase with catalytic properties altered during reactivation. 3. The tech...

Introduction: The role of midbrain reticular formation, which includes the nucleus cuneiformis (NCF), as a crucial antinociceptive region in descending pain modulation has long been investigated. In this study, we tried to highlight the role of NCF in morphine-induced antinociception in formalin-induced pain model in rats. Methods: A total of 201 male Wistar rats weighing 260-310 g were used in...

Most early investigations of the chemistry of endotoxins were limited to gross analyses. I t soon became apparent that subtler means of degradation, such as enzymes, were necessary to determine structural configurations and intramolecular relationships within the endotoxin complex. Just such a subtle endotoxin-degrading system was suggested by the observation of Hegemann (1954) that human plasm...

Rickettsiae that have been frozen and thawed in isotonic salt solutions show greatly decreased toxicity for mice, hemolytic activity, respiration, and infectivity for eggs. All these properties can be partially restored by incubation of the rickettsiae in the presence of DPN and coenzyme A for 2 hours at 34 degrees C. The extent of both inactivation and of subsequent reactivation is markedly af...