The concentrations of riboflavin and of the two coenzymes derived from riboflavin, flavin mononucleotide and flavin adenine dinucleotide (FAD), were determined in Novikoff hepatoma grown i.p. and in host liver of normal and riboflavin-deficient male Holtzman rats. Riboflavin deficiency significantly prolonged the 50% survival time of tumor-bearing animals from 5.7 to 10.8 days. The growth of No...

BACKGROUND Discrepancies have been reported between estimates of the prevalence of riboflavin deficiency based on intakes of riboflavin and estimates based on measures of riboflavin status. One reason for this may be an overestimate of the bioavailability of riboflavin from foods, about which relatively little is known. OBJECTIVE We aimed to quantify the bioavailability of riboflavin from mil...

Hypertension, defined as a systolic/diastolic blood pressure of 140/90 mmHg or greater, is estimated to carry a three-fold increased risk of developing cardiovascular diseases (CVDs). Evidence from genome-wide association studies has identified an association between blood pressure and the gene encoding the folate-metabolising enzyme, methylenetetrahydrofolate reductase (MTHFR). Recent meta-ana...

BACKGROUND The industrial production of riboflavin mostly relies on the microbial fermentation of flavinogenic microorganisms and Ashbya gossypii is the main industrial producer of the vitamin. Accordingly, bioengineering strategies aimed at increasing riboflavin production in A. gossypii are highly valuable for industry. RESULTS We analyze the contribution of all the RIB genes to the product...

Since new analogues of riboflavin have been synthesized which inhibit the growth of rats and of microorganisms which require riboflavin (l-3), it was decided to test the effect of some of these compounds upon the utilization of riboflavin and flavin adenine dinucleotide (FAD) for growth of Lactobacillus casei. Other compounds structurally related to riboflavin were also studied. It has been sho...

Corneal collagen cross-linking (CXL) has been described as a promising therapy for keratoconus. According to standard CXL protocol, epithelium should be debrided before treatment to allow penetration of riboflavin into the corneal stroma. However, removal of the epithelium can increase procedure risks. In this study we aim to evaluate stromal penetration of a biocompatible riboflavin-based nano...

Riboflavin secretion by Hyoscyamus albus hairy roots under Fe deficiency was examined to determine where riboflavin is produced and whether production occurs via an enhancement of riboflavin biosynthesis or a stimulation of flavin mononucleotide (FMN) hydrolysis. Confocal fluorescent microscopy showed that riboflavin was mainly localized in the epidermis and cortex of the root tip and, at the c...

Herein, fast Fourier transformation square-wave voltammetry (FFT-SWV) as a novel electrochemical determination technique was used to investigate the electrochemical behavior and determination of Riboflavin at the surface of a nanocomposite modified carbon paste electrode. The carbon paste electrode was modified by nanocomposite containing Samarium oxide (Sm2O3)/reduced gra...

The erythrocyte antioxidant enzyme levels of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of riboflavin-deficient and Trichinella spiralis-infected rats were investigated. The rats were deprived of riboflavin at the 8th week of the experiment. At that time, the erythrocyte glutathione reductase activity coefficient (EGR AC), as an indicator of riboflavin status, was ...

The chemical syntheses of monoand dimethyl esters of riboflavin phosphate were accomplished by allowing riboflavin to react with the appropriate monoand dimethylchlorophosphoric acids. Riboflavin monomethyl phosphate was found to be coenzymatically active with nicotinamide adenine dinucleotide phosphate-cytochrome c aporeductase, pyridoxine (or pyridoxamine) phosphate apooxidase, and glycolate ...