Background: T-helper cells play an essential role in the progression of lymphedema. This study aimed to explore biological significance cell-associated genes (THAGs) a mouse tail model lymphedema by RNA-sequencing (RNA-seq) data.

Sequencing of RNA has been an important application of DNA sequencing technology since its invention. RNA is usually sequenced by first converting it to complementary DNA (cDNA) with the reverse transcriptase enzyme (RNA-dependent DNA polymerase). Reverse transcriptase was originally isolated from Rous sarcoma retrovirus and Rauscher mouse leukemia retrovirus (R-MLV) by Baltimore (1970) and ind...

Current RNA expression profiling methods rely on enrichment steps for specific RNA classes, thereby not detecting all RNA species in an unperturbed manner. We report strand-specific RNAome sequencing that determines expression of small and large RNAs from rRNA-depleted total RNA in a single sequence run. Since current analysis pipelines cannot reliably analyze small and large RNAs simultaneousl...

Schizophrenia (SCZ) is a serious psychiatric disorder that affects 1% of general population and places a heavy burden worldwide. The underlying genetic mechanism of SCZ remains unknown, but studies indicate that the disease is associated with a global gene expression disturbance across many genes. Next-generation sequencing, particularly of RNA sequencing (RNA-Seq), provides a powerful genome-s...

Precision-cut lung slices (PCLS) have gained increasing interest as a model to study biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of compared with animal models. Several experimental readouts been established for use PCLS, but obtaining high-yield -quality RNA downstream analysis has remained challenging. Th...

Analyzing EGFR mutations and detecting ALK gene fusion are indispensable when planning to treat pulmonary adenocarcinoma. Malignant pleural effusion (MPE) is a devastating complication of lung cancer and sometimes the only source for mutation analysis. The percentage of tumor cells in the pleural effusion may be low; therefore, mutant enrichment is required for a successful analysis. The EGFR m...

Abstract Purpose of Review Whole exome sequencing (WES) and whole-genome (WGS) are frontline approaches for the genetic diagnosis rare diseases. However, WES/WGS fails in up to 75% cases. Transcriptomics via RNA-sequencing (RNA-Seq) is a novel approach that aims increase diagnostic yield Recent Findings publications focus on success RNA-Seq increasing rates WES/WGS-negative patients 36% cases, ...

Salivary biomarkers for disease detection, diagnostic and prognostic assessments have become increasingly well established in recent years. In this chapter we explain the current leading technology that has been used to characterize salivary non-coding RNAs (ncRNAs) from the extracellular RNA (exRNA) fraction: HiSeq from Illumina® platform for RNA sequencing. Therefore, the chapter is divided i...

Abstract Atherosclerotic plaques form in the inner layer of arteries triggering heart attacks and strokes. Although T cells have been detected atherosclerosis, tolerance dysfunction as a disease driver remains unexplored. Here we examine checkpoints atherosclerotic plaques, artery tertiary lymphoid organs lymph nodes mice burdened by advanced via single-cell RNA sequencing paired with cell anti...