The effect of a bacteriophage cocktail (EcoShield™) that is specific against Escherichia coli O157:H7 was evaluated against a nalidixic acid-resistant enterohemorrhagic E. coli O157:H7 RM4407 (EHEC) strain on leafy greens stored under either (1) ambient air or (2) modified atmosphere (MA; 5% O2/35% CO2/60% N2). Pieces (~2 × 2 cm2) of leafy greens (lettuce and spinach) inoculated with 4.5 log CF...

Carotenoids in the photosynthetic membranes of plants typically contain two b-rings (e.g., b-carotene and zeaxanthin) or one «and one b-ring (e.g., lutein). Carotenoids with two «-rings are uncommon. We reported earlier that the Arabidopsis thaliana lycopene «-cyclase (LCYe) adds one «-ring to the symmetrical linear substrate lycopene, whereas the structurally related lycopene b-cyclase (LCYb) ...

Escherichia coli O145:H28 strain RM12581 was isolated from bagged romaine lettuce during a 2010 U.S. lettuce-associated outbreak. E. coli O145:H28 strain RM12761 was isolated from ice cream during a 2007 ice cream-associated outbreak in Belgium. Here we report the complete genome sequences and annotation of both strains.

Abstract The plastic tunnel can ensure a positive effect on the yield and quality of vegetables. In our experiment, we evaluated following types varieties lettuce – heading type (‘Great Lakes 659’, ‘King May’), non-heading (‘Lollo Rossa’, ‘Lollo Bionda’), cos (‘Romaine lettuce’). highest head weight was detected in ‘Great 659’ (373.97 g plant −1 ) ‘Romaine lettuce’ (266.79 under an unheated spr...

RNA prepared from fractions of chloroplasts and mitochondria sedimented at rates characteristic of ribosomal RNA. A predominance of the 18S species was frequently observed in preparations from chloroplasts from romaine lettuce and endive. The usual distribution, a preponderance of the 28S species, was observed in studies on tomato and spinach chloroplasts and mitochondria from mushroom and caul...

Pathogenic bacteria that become internalized in leaf tissues are protected from the antimicrobial effects of surface treatments. Ionizing radiation is known to penetrate food tissues, but the efficacy of the process against internalized bacteria is unknown. Leaves of Romaine lettuce and baby spinach were cut into pieces, submerged in a cocktail mixture of three isolates of Escherichia coli O157...

Romaine and Boston lettuce (Lactuca sativa L., Compositae) were identified as economically feasible alternative crops for eastern Virginia (Sterrett et al. 1996; Kalo 1997). Although lettuce is one of the most widely consumed vegetables, much of the production is concentrated in western states. Per capita consumption of leaf lettuce has increased from a low of 1.1 kg in 1986 to an estimated 3.4...

This paper reported the development of hyperspectral fluorescence imaging system using ultraviolet-A excitation (320400 nm) for detection of bovine fecal contaminants on the abaxial and adaxial surfaces of romaine lettuce and baby spinach leaves. Six spots of fecal contamination were applied to each of 40 lettuce and 40 spinach leaves. In this study, the wavebands at 666 nm and 680 nm were sele...

food preservation has always been a concern for mankind. different methods have been developed to preserve food for long periods, such as cold storage, plastic covers, and modified atmospherethis study examined the physicochemical and biological effects of a modified atmosphere on packaged romaine lettuce at three levels (g1: 40% oxygen, 15% carbon dioxide, 45% nitrogen; g2: 70% oxygen, 15% car...

Leafy vegetables, such as lettuce ( Lactuca sativa L), have been identified as a potential source of perchlorate exposure to humans. Perchlorate is of concern because excessive amounts may impair thyroid function by inhibiting iodide uptake by the sodium iodide symporter. Perchlorate has been identified as an oxidation product in sodium hypochlorite. Dilute hypochlorite solutions are widely use...